Human PYCARD ELISA Kit

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  • Alternative name

    Human hASC ELISA Kit;Human caspase recruitment domain-containing protein 5 ELISA Kit;Human PYD and CARD domain-containing protein ELISA Kit;Human target of methylation-induced silencing 1 ELISA Kit;Human ASC ELISA Kit;Human CARD5 ELISA Kit;Human TMS1 ELISA Kit;Human TMS ELISA Kit;Human TMS-1 ELISA Kit;Human PYD and CARD domain containing ELISA Kit;Human apoptosis-associated speck-like protein containing a CARD ELISA Kit;

  • Catalog
    E003394
  • species
    Human
  • GenePYCARD
  • Standard CurveHuman PYCARD ELISA Kit
  • Other Species Mouse Pycard ELISA KitBovine PYCARD ELISA KitHuman PYCARD-AS1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PYCARD. No significant cross-reactivity or interference between PYCARD and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PYCARD and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman PYCARD ELISA Kit allows for the in vitro quantitative determination of PYCARD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Principle of the assay: PYCARD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PYCARD antibody and an PYCARD-HRP conjugate. The assay sample and buffer are incubated together with PYCARD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PYCARD concentration since PYCARD from samples and PYCARD-HRP conjugate compete for the anti-PYCARD antibody binding site. Since the number of sites is limited, as more sites are occupied by PYCARD from the sample, fewer sites are left to bind PYCARD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PYCARD concentration in each sample is interpolated from this standard curve.
  • Human Apoptosis-associated speck-like protein containing a CARD Protein information
  • Uniprot ID ASC_HUMAN
  • Uniprot AC Q9ULZ3; Q96D12; Q9BSZ5; Q9HBD0; Q9NXJ8;
  • UniGene Hs.499094;
  • GeneID 29108
  • KEGG hsa:29108;
  • Human Apoptosis-associated speck-like protein containing a CARD Protein SEQUENCE
  • SEQUENCE 195 AA; 21627 MW; 455987286586F46A CRC64;

    MGRARDAILD ALENLTAEEL KKFKLKLLSV PLREGYGRIP RGALLSMDAL

    DLTDKLVSFY LETYGAELTA NVLRDMGLQE MAGQLQAATH QGSGAAPAGI

    QAPPQSAAKP GLHFIDQHRA ALIARVTNVE WLLDALYGKV LTDEQYQAVR

    AEPTNPSKMR KLFSFTPAWN WTCKDLLLQA LRESQSYLVE DLERS

  • UCSC uc002ebm.4; human. [Q9ULZ3-1];


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