Human RBM24 ELISA Kit

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  • Alternative name

    Human RNA-binding motif protein 24 ELISA Kit;Human RNA-binding region-containing protein 6 ELISA Kit;Human RNPC6 ELISA Kit;Human dJ259A10.1 ELISA Kit;Human RNA binding motif protein 24 ELISA Kit;Human RNA-binding protein 24 ELISA Kit;Human RNA-binding region (RNP1, RRM) containing 6 ELISA Kit;

  • Catalog
    E033905
  • species
    Human
  • GeneRBM24
  • Standard CurveHuman RBM24 ELISA Kit
  • Other Species Mouse Rbm24 ELISA KitChicken RBM24 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of RBM-24. No significant cross-reactivity or interference between RBM-24 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RBM-24 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman RBM24 ELISA Kit allows for the in vitro quantitative determination of RBM24 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses||This RBM-24 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of HumanRBM-24. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: RBM-24 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RBM-24 antibody and an RBM-24-HRP conjugate. The assay sample and buffer are incubated together with RBM-24-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RBM-24 concentration since RBM-24 from samples and RBM-24-HRP conjugate compete for the anti-RBM-24 antibody binding site. Since the number of sites is limited, as more sites are occupied by RBM-24 from the sample, fewer sites are left to bind RBM-24-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RBM-24 concentration in each sample is interpolated from this standard curve.
  • Human RNA-binding protein 24 Protein information
  • Uniprot ID RBM24_HUMAN
  • Uniprot AC Q9BX46; E9PAY4; Q6QDA4; Q8N9D3; Q96NI3;
  • UniGene Hs.519904;
  • GeneID 221662
  • KEGG hsa:221662;
  • Human RNA-binding protein 24 Protein SEQUENCE
  • SEQUENCE 236 AA; 24776 MW; 1CFB5AEBD4E3AA24 CRC64;

    MHTTQKDTTY TKIFVGGLPY HTTDASLRKY FEVFGEIEEA VVITDRQTGK

    SRGYGFVTMA DRAAAERACK DPNPIIDGRK ANVNLAYLGA KPRIMQPGFA

    FGVQQLHPAL IQRPFGIPAH YVYPQAFVQP GVVIPHVQPT AAAASTTPYI

    DYTGAAYAQY SAAAAAAAAA AAYDQYPYAA SPAAAGYVTA GGYGYAVQQP

    ITAAAPGTAA AAAAAAAAAA AFGQYQPQQL QTDRMQ

  • UCSC uc003nbz.5; human. [Q9BX46-1];


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