Human RIOK1 ELISA Kit

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  • Alternative name

    Human RIO kinase 1 ELISA Kit;Human AD034 ELISA Kit;Human RRP10 ELISA Kit;Human bA288G3.1 ELISA Kit;Human serine/threonine-protein kinase RIO1 ELISA Kit;

  • Catalog
    E033835
  • species
    Human
  • GeneRIOK1
  • Standard CurveHuman RIOK1 ELISA Kit
  • Other Species Mouse Riok1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of RIO Kinase 1 (RIOK1). No significant cross-reactivity or interference between RIO Kinase 1 (RIOK1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.133ng/mL.
  • Intended UseHuman RIOK1 ELISA Kit allows for the in vitro quantitative determination of RIOK1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RIO Kinase 1 (RIOK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RIO Kinase 1 (RIOK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RIO Kinase 1 (RIOK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RIO Kinase 1 (RIOK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Serine/threonine-protein kinase RIO1 Protein information
  • Uniprot ID RIOK1_HUMAN
  • Uniprot AC Q9BRS2; B2RB28; Q8NDC8; Q96NV9;
  • UniGene Hs.437474;
  • GeneID 83732
  • KEGG hsa:83732;
  • Human Serine/threonine-protein kinase RIO1 Protein SEQUENCE
  • SEQUENCE 568 AA; 65583 MW; 5730BFC3798F5190 CRC64;

    MDYRRLLMSR VVPGQFDDAD SSDSENRDLK TVKEKDDILF EDLQDNVNEN

    GEGEIEDEEE EGYDDDDDDW DWDEGVGKLA KGYVWNGGSN PQANRQTSDS

    SSAKMSTPAD KVLRKFENKI NLDKLNVTDS VINKVTEKSR QKEADMYRIK

    DKADRATVEQ VLDPRTRMIL FKMLTRGIIT EINGCISTGK EANVYHASTA

    NGESRAIKIY KTSILVFKDR DKYVSGEFRF RHGYCKGNPR KMVKTWAEKE

    MRNLIRLNTA EIPCPEPIML RSHVLVMSFI GKDDMPAPLL KNVQLSESKA

    RELYLQVIQY MRRMYQDARL VHADLSEFNM LYHGGGVYII DVSQSVEHDH

    PHALEFLRKD CANVNDFFMR HSVAVMTVRE LFEFVTDPSI THENMDAYLS

    KAMEIASQRT KEERSSQDHV DEEVFKRAYI PRTLNEVKNY ERDMDIIMKL

    KEEDMAMNAQ QDNILYQTVT GLKKDLSGVQ KVPALLENQV EERTCSDSED

    IGSSECSDTD SEEQGDHARP KKHTTDPDID KKERKKMVKE AQREKRKNKI

    PKHVKKRKEK TAKTKKGK

  • UCSC uc003mxn.4; human.;


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