Human RNASE1 ELISA Kit

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  • Alternative name

    Human HP-RNase ELISA Kit;Human RIB-1 ELISA Kit;Human RNase upI-1 ELISA Kit;Human ribonuclease 1 ELISA Kit;Human RNase 1 ELISA Kit;Human Ribonuclease A ELISA Kit;Human RNase A ELISA Kit;Human RIB1 ELISA Kit;Human RNS1 ELISA Kit;Human RAC1 ELISA Kit;Human ribonuclease A family member 1, pancreatic ELISA Kit;Human ribonuclease pancreatic ELISA Kit;Human ribonuclease A C1 ELISA Kit;Human ribonuclease, RNase A family, 1 (pancreatic) ELISA Kit;

  • Catalog
    E033592
  • species
    Human
  • GeneRNASE1
  • Standard CurveHuman RNASE1 ELISA Kit
  • Other Species Mouse Rnase1 ELISA KitBovine RNASE1 ELISA KitGoat RNASE1 ELISA KitHamster RNASE1 ELISA KitHorse RNASE1 ELISA KitPorcine RNASE1 ELISA KitSheep RNASE1 ELISA KitRat Rnase1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Ribonuclease A (RNASE1). No significant cross-reactivity or interference between Ribonuclease A (RNASE1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 30pg/mL.
  • Intended UseHuman RNASE1 ELISA Kit allows for the in vitro quantitative determination of RNASE1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease A (RNASE1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease A (RNASE1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease A (RNASE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ribonuclease A (RNASE1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Ribonuclease pancreatic Protein information
  • Uniprot ID RNAS1_HUMAN
  • Uniprot AC Q9UCB5;
  • UniGene Hs.78224;
  • GeneID 6035
  • KEGG hsa:6035;
  • Human Ribonuclease pancreatic Protein SEQUENCE
  • SEQUENCE 156 AA; 17644 MW; F63B17B8B55115F9 CRC64;

    MALEKSLVRL LLLVLILLVL GWVQPSLGKE SRAKKFQRQH MDSDSSPSSS

    STYCNQMMRR RNMTQGRCKP VNTFVHEPLV DVQNVCFQEK VTCKNGQGNC

    YKSNSSMHIT DCRLTNGSRY PNCAYRTSPK ERHIIVACEG SPYVPVHFDA

    SVEDST



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