Human HP-RNase ELISA Kit;Human RIB-1 ELISA Kit;Human RNase upI-1 ELISA Kit;Human ribonuclease 1 ELISA Kit;Human RNase 1 ELISA Kit;Human Ribonuclease A ELISA Kit;Human RNase A ELISA Kit;Human RIB1 ELISA Kit;Human RNS1 ELISA Kit;Human RAC1 ELISA Kit;Human ribonuclease A family member 1, pancreatic ELISA Kit;Human ribonuclease pancreatic ELISA Kit;Human ribonuclease A C1 ELISA Kit;Human ribonuclease, RNase A family, 1 (pancreatic) ELISA Kit;
Catalog
E033592
species
Human
GeneRNASE1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Ribonuclease A (RNASE1).
No significant cross-reactivity or interference between Ribonuclease A (RNASE1) and analogues was observed.
Intended UseHuman RNASE1 ELISA Kit allows for the in vitro quantitative determination of RNASE1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease A (RNASE1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease A (RNASE1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease A (RNASE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ribonuclease A (RNASE1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.