Human RHO ELISA Kit

Alternative name
Human opsin-2 ELISA Kit;Human OPN2 ELISA Kit;Human CSNBAD1 ELISA Kit;Human RP4 ELISA Kit;Human rhodopsin ELISA Kit;Human opsin 2, rod pigment ELISA Kit;
Catalog
E033558
species
Human
GeneRHO
SpecificityThis assay has high sensitivity and excellent specificity for detection of Rhodopsin (RHO). No significant cross-reactivity or interference between Rhodopsin (RHO) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.64ng/mL.
Intended UseHuman RHO ELISA Kit allows for the in vitro quantitative determination of RHO , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rhodopsin (RHO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Rhodopsin (RHO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rhodopsin (RHO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rhodopsin (RHO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Rhodopsin Protein information
Uniprot ID OPSD_HUMAN
Uniprot AC P08100; Q16414; Q2M249;
UniGene Hs.247565;
GeneID 6010
KEGG hsa:6010;
Human Rhodopsin Protein SEQUENCE
SEQUENCE 348 AA; 38893 MW; 6F4F6FCBA34265B2 CRC64;
MNGTEGPNFY VPFSNATGVV RSPFEYPQYY LAEPWQFSML AAYMFLLIVL
GFPINFLTLY VTVQHKKLRT PLNYILLNLA VADLFMVLGG FTSTLYTSLH
GYFVFGPTGC NLEGFFATLG GEIALWSLVV LAIERYVVVC KPMSNFRFGE
NHAIMGVAFT WVMALACAAP PLAGWSRYIP EGLQCSCGID YYTLKPEVNN
ESFVIYMFVV HFTIPMIIIF FCYGQLVFTV KEAAAQQQES ATTQKAEKEV
TRMVIIMVIA FLICWVPYAS VAFYIFTHQG SNFGPIFMTI PAFFAKSAAI
YNPVIYIMMN KQFRNCMLTT ICCGKNPLGD DEASATVSKT ETSQVAPA
UCSC uc003emt.4; human.;

Category

Human RHO ELISA Kit

Human opsin-2 ELISA Kit;Human OPN2 ELISA Kit;Human CSNBAD1 ELISA Kit;Human RP4 ELISA Kit;Human rhodopsin ELISA Kit;Human opsin 2, rod pigment ELISA Kit;