Human RHO ELISA Kit

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  • Alternative name

    Human opsin-2 ELISA Kit;Human OPN2 ELISA Kit;Human CSNBAD1 ELISA Kit;Human RP4 ELISA Kit;Human rhodopsin ELISA Kit;Human opsin 2, rod pigment ELISA Kit;

  • Catalog
    E033558
  • species
    Human
  • GeneRHO
  • Standard CurveHuman RHO ELISA Kit
  • Other Species Mouse Rho ELISA KitChicken Hemoglobin subunit rho ELISA KitBovine RHO ELISA KitCanine RHO ELISA KitChicken RHO ELISA KitHamster RHO ELISA KitMonkey RHO ELISA KitPorcine RHO ELISA KitRabbit RHO ELISA KitRat Rho ELISA KitSheep RHO ELISA KitRat Rho-GTPase-activating protein ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Rhodopsin (RHO). No significant cross-reactivity or interference between Rhodopsin (RHO) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.64ng/mL.
  • Intended UseHuman RHO ELISA Kit allows for the in vitro quantitative determination of RHO , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rhodopsin (RHO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Rhodopsin (RHO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rhodopsin (RHO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rhodopsin (RHO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Rhodopsin Protein information
  • Uniprot ID OPSD_HUMAN
  • Uniprot AC P08100; Q16414; Q2M249;
  • UniGene Hs.247565;
  • GeneID 6010
  • KEGG hsa:6010;
  • Human Rhodopsin Protein SEQUENCE
  • SEQUENCE 348 AA; 38893 MW; 6F4F6FCBA34265B2 CRC64;

    MNGTEGPNFY VPFSNATGVV RSPFEYPQYY LAEPWQFSML AAYMFLLIVL

    GFPINFLTLY VTVQHKKLRT PLNYILLNLA VADLFMVLGG FTSTLYTSLH

    GYFVFGPTGC NLEGFFATLG GEIALWSLVV LAIERYVVVC KPMSNFRFGE

    NHAIMGVAFT WVMALACAAP PLAGWSRYIP EGLQCSCGID YYTLKPEVNN

    ESFVIYMFVV HFTIPMIIIF FCYGQLVFTV KEAAAQQQES ATTQKAEKEV

    TRMVIIMVIA FLICWVPYAS VAFYIFTHQG SNFGPIFMTI PAFFAKSAAI

    YNPVIYIMMN KQFRNCMLTT ICCGKNPLGD DEASATVSKT ETSQVAPA

  • UCSC uc003emt.4; human.;



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