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Human RHOB ELISA Kit

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  • Alternative name

    Human rho cDNA clone 6 ELISA Kit;Human h6 ELISA Kit;Human ARH6 ELISA Kit;Human ARHB ELISA Kit;Human MST081 ELISA Kit;Human MSTP081 ELISA Kit;Human RHOH6 ELISA Kit;Human ras homolog family member B ELISA Kit;Human rho-related GTP-binding protein RhoB ELISA Kit;Human Aplysia RAS-related homolog 6 ELISA Kit;Human oncogene RHO H6 ELISA Kit;Human ras homolog gene family, member B ELISA Kit;
  • Catalog
    E033554
  • species
    Human
  • GeneRHOB
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human RHOB. No significant cross-reactivity or interference between Human RHOB and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human RHOB and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman RHOB ELISA Kit allows for the in vitro quantitative determination of RHOB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of RHOB in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RHOB. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human RHOB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RHOB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human RHOB. You can calculate the concentration of Human RHOB in the samples by comparing the OD of the samples to the standard curve.
  • Human Rho-related GTP-binding protein RhoB Protein information
  • Uniprot ID RHOB_HUMAN
  • Uniprot AC P62745; B2R692; P01121; Q5U0H6; Q7RTN5; Q7RTR9; Q9CUV7;
  • UniGene Hs.502876;
  • GeneID 388
  • KEGG hsa:388;
  • Human Rho-related GTP-binding protein RhoB Protein SEQUENCE

  • SEQUENCE 196 AA; 22123 MW; CCE6FD53AE00CD83 CRC64;

    MAAIRKKLVV VGDGACGKTC LLIVFSKDEF PEVYVPTVFE NYVADIEVDG

    KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSVDSPD SLENIPEKWV

    PEVKHFCPNV PIILVANKKD LRSDEHVRTE LARMKQEPVR TDDGRAMAVR

    IQAYDYLECS AKTKEGVREV FETATRAALQ KRYGSQNGCI NCCKVL

  • UCSC uc002rdv.4; human.;



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