Human RFC1 ELISA Kit

Contact Us
  • Alternative name

    Human activator 1 140 kDa subunit ELISA Kit;Human A1 140 kDa subunit ELISA Kit;Human activator 1 large subunit ELISA Kit;Human activator 1 subunit 1 ELISA Kit;Human DNA-binding protein PO-GA ELISA Kit;Human replication factor C 140 kDa subunit ELISA Kit;Human RF-C 140 kDa subunit ELISA Kit;Human RFC140 ELISA Kit;Human replication factor C large subunit ELISA Kit;Human A1 ELISA Kit;Human MHCBFB ELISA Kit;Human PO-GA ELISA Kit;Human RECC1 ELISA Kit;Human RFC ELISA Kit;Human replication factor C subunit 1 ELISA Kit;Human MHC binding factor, beta ELISA Kit;Human replication factor C (activator 1) 1, 145kDa ELISA Kit;Human replication factor C1 ELISA Kit;

  • Catalog
    E033446
  • species
    Human
  • GeneRFC1
  • Standard CurveHuman RFC1 ELISA Kit
  • Other Species Mouse Rfc1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of RFC1 . No significant cross-reactivity or interference between RFC1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 18.75pg/ml
  • Intended UseHuman RFC1 ELISA Kit allows for the in vitro quantitative determination of RFC1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- RFC1antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- RFC1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the RFC1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of RFC1 can be calculated.
  • Human Replication factor C subunit 1 Protein information
  • Uniprot ID RFC1_HUMAN
  • Uniprot AC P35251; A8K6E7; Q5XKF5; Q6PKU0; Q86V41; Q86V46;
  • UniGene Hs.507475;
  • GeneID 5981
  • KEGG hsa:5981;
  • Human Replication factor C subunit 1 Protein SEQUENCE
  • SEQUENCE 1148 AA; 128255 MW; 485F0332FB56819B CRC64;

    MDIRKFFGVI PSGKKLVSET VKKNEKTKSD EETLKAKKGI KEIKVNSSRK

    EDDFKQKQPS KKKRIIYDSD SESEETLQVK NAKKPPEKLP VSSKPGKISR

    QDPVTYISET DEEDDFMCKK AASKSKENGR STNSHLGTSN MKKNEENTKT

    KNKPLSPIKL TPTSVLDYFG TGSVQRSNKK MVASKRKELS QNTDESGLND

    EAIAKQLQLD EDAELERQLH EDEEFARTLA MLDEEPKTKK ARKDTEAGET

    FSSVQANLSK AEKHKYPHKV KTAQVSDERK SYSPRKQSKY ESSKESQQHS

    KSSADKIGEV SSPKASSKLA IMKRKEESSY KEIEPVASKR KENAIKLKGE

    TKTPKKTKSS PAKKESVSPE DSEKKRTNYQ AYRSYLNREG PKALGSKEIP

    KGAENCLEGL IFVITGVLES IERDEAKSLI ERYGGKVTGN VSKKTNYLVM

    GRDSGQSKSD KAAALGTKII DEDGLLNLIR TMPGKKSKYE IAVETEMKKE

    SKLERTPQKN VQGKRKISPS KKESESKKSR PTSKRDSLAK TIKKETDVFW

    KSLDFKEQVA EETSGDSKAR NLADDSSENK VENLLWVDKY KPTSLKTIIG

    QQGDQSCANK LLRWLRNWQK SSSEDKKHAA KFGKFSGKDD GSSFKAALLS

    GPPGVGKTTT ASLVCQELGY SYVELNASDT RSKSSLKAIV AESLNNTSIK

    GFYSNGAASS VSTKHALIMD EVDGMAGNED RGGIQELIGL IKHTKIPIIC

    MCNDRNHPKI RSLVHYCFDL RFQRPRVEQI KGAMMSIAFK EGLKIPPPAM

    NEIILGANQD IRQVLHNLSM WCARSKALTY DQAKADSHRA KKDIKMGPFD

    VARKVFAAGE ETAHMSLVDK SDLFFHDYSI APLFVQENYI HVKPVAAGGD

    MKKHLMLLSR AADSICDGDL VDSQIRSKQN WSLLPAQAIY ASVLPGELMR

    GYMTQFPTFP SWLGKHSSTG KHDRIVQDLA LHMSLRTYSS KRTVNMDYLS

    LLRDALVQPL TSQGVDGVQD VVALMDTYYL MKEDFENIME ISSWGGKPSP

    FSKLDPKVKA AFTRAYNKEA HLTPYSLQAI KASRHSTSPS LDSEYNEELN

    EDDSQSDEKD QDAIETDAMI KKKTKSSKPS KPEKDKEPRK GKGKSSKK

  • UCSC uc003gtx.3; human. [P35251-1];



Inquiry About Human RFC1 ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!