Human RFC1 ELISA Kit

Alternative name
Human activator 1 140 kDa subunit ELISA Kit;Human A1 140 kDa subunit ELISA Kit;Human activator 1 large subunit ELISA Kit;Human activator 1 subunit 1 ELISA Kit;Human DNA-binding protein PO-GA ELISA Kit;Human replication factor C 140 kDa subunit ELISA Kit;Human RF-C 140 kDa subunit ELISA Kit;Human RFC140 ELISA Kit;Human replication factor C large subunit ELISA Kit;Human A1 ELISA Kit;Human MHCBFB ELISA Kit;Human PO-GA ELISA Kit;Human RECC1 ELISA Kit;Human RFC ELISA Kit;Human replication factor C subunit 1 ELISA Kit;Human MHC binding factor, beta ELISA Kit;Human replication factor C (activator 1) 1, 145kDa ELISA Kit;Human replication factor C1 ELISA Kit;
Catalog
E033446
species
Human
GeneRFC1
SpecificityThis assay has high sensitivity and excellent specificity for detection of RFC1 . No significant cross-reactivity or interference between RFC1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 18.75pg/ml
Intended UseHuman RFC1 ELISA Kit allows for the in vitro quantitative determination of RFC1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- RFC1antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- RFC1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the RFC1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of RFC1 can be calculated.
Human Replication factor C subunit 1 Protein information
Uniprot ID RFC1_HUMAN
Uniprot AC P35251; A8K6E7; Q5XKF5; Q6PKU0; Q86V41; Q86V46;
UniGene Hs.507475;
GeneID 5981
KEGG hsa:5981;
Human Replication factor C subunit 1 Protein SEQUENCE
SEQUENCE 1148 AA; 128255 MW; 485F0332FB56819B CRC64;
MDIRKFFGVI PSGKKLVSET VKKNEKTKSD EETLKAKKGI KEIKVNSSRK
EDDFKQKQPS KKKRIIYDSD SESEETLQVK NAKKPPEKLP VSSKPGKISR
QDPVTYISET DEEDDFMCKK AASKSKENGR STNSHLGTSN MKKNEENTKT
KNKPLSPIKL TPTSVLDYFG TGSVQRSNKK MVASKRKELS QNTDESGLND
EAIAKQLQLD EDAELERQLH EDEEFARTLA MLDEEPKTKK ARKDTEAGET
FSSVQANLSK AEKHKYPHKV KTAQVSDERK SYSPRKQSKY ESSKESQQHS
KSSADKIGEV SSPKASSKLA IMKRKEESSY KEIEPVASKR KENAIKLKGE
TKTPKKTKSS PAKKESVSPE DSEKKRTNYQ AYRSYLNREG PKALGSKEIP
KGAENCLEGL IFVITGVLES IERDEAKSLI ERYGGKVTGN VSKKTNYLVM
GRDSGQSKSD KAAALGTKII DEDGLLNLIR TMPGKKSKYE IAVETEMKKE
SKLERTPQKN VQGKRKISPS KKESESKKSR PTSKRDSLAK TIKKETDVFW
KSLDFKEQVA EETSGDSKAR NLADDSSENK VENLLWVDKY KPTSLKTIIG
QQGDQSCANK LLRWLRNWQK SSSEDKKHAA KFGKFSGKDD GSSFKAALLS
GPPGVGKTTT ASLVCQELGY SYVELNASDT RSKSSLKAIV AESLNNTSIK
GFYSNGAASS VSTKHALIMD EVDGMAGNED RGGIQELIGL IKHTKIPIIC
MCNDRNHPKI RSLVHYCFDL RFQRPRVEQI KGAMMSIAFK EGLKIPPPAM
NEIILGANQD IRQVLHNLSM WCARSKALTY DQAKADSHRA KKDIKMGPFD
VARKVFAAGE ETAHMSLVDK SDLFFHDYSI APLFVQENYI HVKPVAAGGD
MKKHLMLLSR AADSICDGDL VDSQIRSKQN WSLLPAQAIY ASVLPGELMR
GYMTQFPTFP SWLGKHSSTG KHDRIVQDLA LHMSLRTYSS KRTVNMDYLS
LLRDALVQPL TSQGVDGVQD VVALMDTYYL MKEDFENIME ISSWGGKPSP
FSKLDPKVKA AFTRAYNKEA HLTPYSLQAI KASRHSTSPS LDSEYNEELN
EDDSQSDEKD QDAIETDAMI KKKTKSSKPS KPEKDKEPRK GKGKSSKK
UCSC uc003gtx.3; human. [P35251-1];

Category

Human RFC1 ELISA Kit