Human RECK ELISA Kit

Alternative name
Human hRECK ELISA Kit;Human suppressor of tumorigenicity 15 protein ELISA Kit;Human ST15 ELISA Kit;Human reversion inducing cysteine rich protein with kazal motifs ELISA Kit;Human reversion-inducing cysteine-rich protein with Kazal motifs ELISA Kit;Human membrane-anchored glycoprotein (metastasis and invasion) ELISA Kit;Human suppression of tumorigenicity 15 (reversion-inducing-cysteine-rich protein with kazal motifs) ELISA Kit;Human suppression of tumorigenicity 5 (reversion-inducing-cysteine-rich protein with kazal motifs) ELISA Kit;
Catalog
E033440
species
Human
GeneRECK
SpecificityThis assay has high sensitivity and excellent specificity for detection of human RECK. No significant cross-reactivity or interference between human RECK and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human RECK is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman RECK ELISA Kit allows for the in vitro quantitative determination of RECK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RECK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RECK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RECK is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RECK bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Reversion-inducing cysteine-rich protein with Kazal motifs Protein information
Uniprot ID RECK_HUMAN
Uniprot AC NKEAEKIE SLINSDSPTL ASHVPLSALI ISQVQVSSSV PSAGVRARPS CHSLLLPLSL
UniGene Hs.388918;
GeneID 8434
KEGG hsa:8434;
Human Reversion-inducing cysteine-rich protein with Kazal motifs Protein SEQUENCE
SEQUENCE 971 AA; 106457 MW; 173D47D6AEE6F834 CRC64;
MATVRASLRG ALLLLLAVAG VAEVAGGLAP GSAGALCCNH SKDNQMCRDV
CEQIFSSKSE SRLKHLLQRA PDYCPETMVE IWNCMNSSLP GVFKKSDGWV
GLGCCELAIA LECRQACKQA SSKNDISKVC RKEYENALFS CISRNEMGSV
CCSYAGHHTN CREYCQAIFR TDSSPGPSQI KAVENYCASI SPQLIHCVNN
YTQSYPMRNP TDSLYCCDRA EDHACQNACK RILMSKKTEM EIVDGLIEGC
KTQPLPQDPL WQCFLESSQS VHPGVTVHPP PSTGLDGAKL HCCSKANTST
CRELCTKLYS MSWGNTQSWQ EFDRFCEYNP VEVSMLTCLA DVREPCQLGC
RNLTYCTNFN NRPTELFRSC NAQSDQGAMN DMKLWEKGSI KMPFINIPVL
DIKKCQPEMW KAIACSLQIK PCHSKSRGSI ICKSDCVEIL KKCGDQNKFP
EDHTAESICE LLSPTDDLKN CIPLDTYLRP STLGNIVEEV THPCNPNPCP
ANELCEVNRK GCPSGDPCLP YFCVQGCKLG EASDFIVRQG TLIQVPSSAG
EVGCYKICSC GQSGLLENCM EMHCIDLQKS CIVGGKRKSH GTSFSIDCNV
CSCFAGNLVC STRLCLSEHS SEDDRRTFTG LPCNCADQFV PVCGQNGRTY
PSACIARCVG LQDHQFEFGS CMSKDPCNPN PCQKNQRCIP KPQVCLTTFD
KFGCSQYECV PRQLACDQVQ DPVCDTDHME HNNLCTLYQR GKSLSYKGPC
QPFCRATEPV CGHNGETYSS VCAAYSDRVA VDYYGDCQAV GVLSEHSSVA
ECASVKCPSL LAAGCKPIIP PGACCPLCAG MLRVLFDKEK LDTIAKVTNK
KPITVLEILQ KIRMHVSVPQ CDVFGYFSIE SEIVILIIPV DHYPKALQIE
ACNKEAEKIE SLINSDSPTL ASHVPLSALI ISQVQVSSSV PSAGVRARPS
CHSLLLPLSL GLALHLLWTY N
UCSC uc003zyv.4; human.;

Category

Human RECK ELISA Kit