Human RBP3 ELISA Kit

Alternative name
Human interphotoreceptor retinoid-binding protein ELISA Kit;Human IRBP ELISA Kit;Human Interstitial retinol-binding protein ELISA Kit;Human D10S64 ELISA Kit;Human D10S65 ELISA Kit;Human D10S66 ELISA Kit;Human RBPI ELISA Kit;Human RP66 ELISA Kit;Human retinol binding protein 3 ELISA Kit;Human retinol-binding protein 3 ELISA Kit;Human interstitial retinol binding protein 3 ELISA Kit;Human retinol binding protein 3, interstitial ELISA Kit;
Catalog
E033392
species
Human
GeneRBP3
SpecificityThis assay has high sensitivity and excellent specificity for detection of RBP3. No significant cross-reactivity or interference between RBP3 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of RBP3 is typically less than 0.055ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two
Intended UseHuman RBP3 ELISA Kit allows for the in vitro quantitative determination of RBP3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RBP3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RBP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RBP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RBP3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Retinol-binding protein 3 Protein information
Uniprot ID RET3_HUMAN
Uniprot AC P10745; Q0QD34; Q5VSR0; Q8IXN0;
UniGene Hs.591928;
GeneID 5949
KEGG hsa:5949;
Human Retinol-binding protein 3 Protein SEQUENCE
SEQUENCE 1247 AA; 135363 MW; 6C1841411E012E0F CRC64;
MMREWVLLMS VLLCGLAGPT HLFQPSLVLD MAKVLLDNYC FPENLLGMQE
AIQQAIKSHE ILSISDPQTL ASVLTAGVQS SLNDPRLVIS YEPSTPEPPP
QVPALTSLSE EELLAWLQRG LRHEVLEGNV GYLRVDSVPG QEVLSMMGEF
LVAHVWGNLM GTSALVLDLR HCTGGQVSGI PYIISYLHPG NTILHVDTIY
NRPSNTTTEI WTLPQVLGER YGADKDVVVL TSSQTRGVAE DIAHILKQMR
RAIVVGERTG GGALDLRKLR IGESDFFFTV PVSRSLGPLG GGSQTWEGSG
VLPCVGTPAE QALEKALAIL TLRSALPGVV HCLQEVLKDY YTLVDRVPTL
LQHLASMDFS TVVSEEDLVT KLNAGLQAAS EDPRLLVRAI GPTETPSWPA
PDAAAEDSPG VAPELPEDEA IRQALVDSVF QVSVLPGNVG YLRFDSFADA
SVLGVLAPYV LRQVWEPLQD TEHLIMDLRH NPGGPSSAVP LLLSYFQGPE
AGPVHLFTTY DRRTNITQEH FSHMELPGPR YSTQRGVYLL TSHRTATAAE
EFAFLMQSLG WATLVGEITA GNLLHTRTVP LLDTPEGSLA LTVPVLTFID
NHGEAWLGGG VVPDAIVLAE EALDKAQEVL EFHQSLGALV EGTGHLLEAH
YARPEVVGQT SALLRAKLAQ GAYRTAVDLE SLASQLTADL QEVSGDHRLL
VFHSPGELVV EEAPPPPPAV PSPEELTYLI EALFKTEVLP GQLGYLRFDA
MAELETVKAV GPQLVRLVWQ QLVDTAALVI DLRYNPGSYS TAIPLLCSYF
FEAEPRQHLY SVFDRATSKV TEVWTLPQVA GQRYGSHKDL YILMSHTSGS
AAEAFAHTMQ DLQRATVIGE PTAGGALSVG IYQVGSSPLY ASMPTQMAMS
ATTGKAWDLA GVEPDITVPM SEALSIAQDI VALRAKVPTV LQTAGKLVAD
NYASAELGAK MATKLSGLQS RYSRVTSEVA LAEILGADLQ MLSGDPHLKA
AHIPENAKDR IPGIVPMQIP SPEVFEELIK FSFHTNVLED NIGYLRFDMF
GDGELLTQVS RLLVEHIWKK IMHTDAMIID MRFNIGGPTS SIPILCSYFF
DEGPPVLLDK IYSRPDDSVS ELWTHAQVVG ERYGSKKSMV ILTSSVTAGT
AEEFTYIMKR LGRALVIGEV TSGGCQPPQT YHVDDTNLYL TIPTARSVGA
SDGSSWEGVG VTPHVVVPAE EALARAKEML QHNQLRVKRS PGLQDHL
UCSC uc001jez.3; human.;

Category

Human RBP3 ELISA Kit