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Human RPE65 ELISA Kit

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  • Alternative name

    Human all-trans-retinyl-palmitate hydrolase ELISA Kit;Human retinal pigment epithelium-specific 65 kDa protein ELISA Kit;Human retinol isomerase ELISA Kit;Human BCO3 ELISA Kit;Human LCA2 ELISA Kit;Human RP20 ELISA Kit;Human mRPE65 ELISA Kit;Human rd12 ELISA Kit;Human sRPE65 ELISA Kit;Human RPE65, retinoid isomerohydrolase ELISA Kit;Human retinoid isomerohydrolase ELISA Kit;Human BCO family, member 3 ELISA Kit;Human RBP-binding membrane protein ELISA Kit;Human p63 ELISA Kit;Human retinal pigment epithelium specific protein 65 ELISA Kit;Human retinal pigment epithelium-specific protein 65kDa ELISA Kit;Human retinitis pigmentosa 20 (autosomal recessive) ELISA Kit;
  • Catalog
    E033378
  • species
    Human
  • GeneRPE65
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of RPE65. No significant cross-reactivity or interference between RPE65 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RPE65 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman RPE65 ELISA Kit allows for the in vitro quantitative determination of RPE65 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: RPE65 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RPE65 antibody and an RPE65-HRP conjugate. The assay sample and buffer are incubated together with RPE65-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RPE65 concentration since RPE65 from samples and RPE65-HRP conjugate compete for the anti-RPE65 antibody binding site. Since the number of sites is limited, as more sites are occupied by RPE65 from the sample, fewer sites are left to bind RPE65-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RPE65 concentration in each sample is interpolated from this standard curve.
  • Human Retinoid isomerohydrolase Protein information
  • Uniprot ID RPE65_HUMAN
  • Uniprot AC Q16518; A8K1L0; Q5T9U3;
  • UniGene Hs.2133;
  • GeneID 6121
  • KEGG hsa:6121;
  • Human Retinoid isomerohydrolase Protein SEQUENCE

  • SEQUENCE 533 AA; 60948 MW; 7193C93F3325798D CRC64;

    MSIQVEHPAG GYKKLFETVE ELSSPLTAHV TGRIPLWLTG SLLRCGPGLF

    EVGSEPFYHL FDGQALLHKF DFKEGHVTYH RRFIRTDAYV RAMTEKRIVI

    TEFGTCAFPD PCKNIFSRFF SYFRGVEVTD NALVNVYPVG EDYYACTETN

    FITKINPETL ETIKQVDLCN YVSVNGATAH PHIENDGTVY NIGNCFGKNF

    SIAYNIVKIP PLQADKEDPI SKSEIVVQFP CSDRFKPSYV HSFGLTPNYI

    VFVETPVKIN LFKFLSSWSL WGANYMDCFE SNETMGVWLH IADKKRKKYL

    NNKYRTSPFN LFHHINTYED NGFLIVDLCC WKGFEFVYNY LYLANLRENW

    EEVKKNARKA PQPEVRRYVL PLNIDKADTG KNLVTLPNTT ATAILCSDET

    IWLEPEVLFS GPRQAFEFPQ INYQKYCGKP YTYAYGLGLN HFVPDRLCKL

    NVKTKETWVW QEPDSYPSEP IFVSHPDALE EDDGVVLSVV VSPGAGQKPA

    YLLILNAKDL SEVARAEVEI NIPVTFHGLF KKS

  • UCSC uc001dei.2; human.;



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