Human RAET1L ELISA Kit

Alternative name
Human ULBP6 ELISA Kit;Human retinoic acid early transcript 1L ELISA Kit;Human retinoic acid early transcript 1L protein ELISA Kit;Human UL16 binding protein 6 ELISA Kit;
Catalog
E033343
species
Human
GeneRAET1L
Other Species Mouse RAET1L ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman RAET1L ELISA Kit allows for the in vitro quantitative determination of RAET1L , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalFor samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This BG RAET1L ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human RAET1L. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY RAET1L ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RAET1L antibody and an RAET1L-HRP conjugate. The assay sample and buffer are incubated together with RAET1L-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RAET1L concentration since RAET1L from samples and RAET1L-HRP conjugate compete for the anti-RAET1L antibody binding site. Since the number of sites is limited, as more sites are occupied by RAET1L from the sample, fewer sites are left to bind RAET1L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RAET1L concentration in each sample is interpolated from this standard curve.
Human Retinoic acid early transcript 1L protein Protein information
Uniprot ID RET1L_HUMAN
Uniprot AC Q5VY80; A3KME4; Q8TE74;
UniGene Hs.558659;
GeneID 154064
KEGG hsa:154064;
Human Retinoic acid early transcript 1L protein Protein SEQUENCE
SEQUENCE 246 AA; 27509 MW; 5FBBEA2289E32690 CRC64;
MAAAAIPALL LCLPLLFLLF GWSRARRDDP HSLCYDITVI PKFRPGPRWC
AVQGQVDEKT FLHYDCGNKT VTPVSPLGKK LNVTMAWKAQ NPVLREVVDI
LTEQLLDIQL ENYTPKEPLT LQARMSCEQK AEGHSSGSWQ FSIDGQTFLL
FDSEKRMWTT VHPGARKMKE KWENDKDVAM SFHYISMGDC IGWLEDFLMG
MDSTLEPSAG APLAMSSGTT QLRATATTLI LCCLLIILPC FILPGI
UCSC uc011eei.2; human.;

Category

Human RAET1L ELISA Kit

Human ULBP6 ELISA Kit;Human retinoic acid early transcript 1L ELISA Kit;Human retinoic acid early transcript 1L protein ELISA Kit;Human UL16 binding protein 6 ELISA Kit;