Human RBL1 ELISA Kit

Alternative name
Human 107 kDa retinoblastoma-associated protein ELISA Kit;Human p107 ELISA Kit;Human PRB1 ELISA Kit;Human CP107 ELISA Kit;Human RB transcriptional corepressor like 1 ELISA Kit;Human retinoblastoma-like protein 1 ELISA Kit;Human retinoblastoma-like 1 ELISA Kit;
Catalog
E033336
species
Human
GeneRBL1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.938ng/ml
Intended UseHuman RBL1 ELISA Kit allows for the in vitro quantitative determination of RBL1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-RBL1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-RBL1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the RBL1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of RBL1 can be calculated.
Human Retinoblastoma-like protein 1 Protein information
Uniprot ID RBL1_HUMAN
Uniprot AC P28749; A8K2W5; Q4VXA0; Q8N5K6; Q9H1L5; Q9H1M1;
UniGene Hs.207745;
GeneID 5933
KEGG hsa:5933;
Human Retinoblastoma-like protein 1 Protein SEQUENCE
SEQUENCE 1068 AA; 120847 MW; 2BC4876EA04BDB31 CRC64;
MFEDKPHAEG AAVVAAAGEA LQALCQELNL DEGSAAEALD DFTAIRGNYS
LEGEVTHWLA CSLYVACRKS IIPTVGKGIM EGNCVSLTRI LRSAKLSLIQ
FFSKMKKWMD MSNLPQEFRE RIERLERNFE VSTVIFKKYE PIFLDIFQNP
YEEPPKLPRS RKQRRIPCSV KDLFNFCWTL FVYTKGNFRM IGDDLVNSYH
LLLCCLDLIF ANAIMCPNRQ DLLNPSFKGL PSDFHTADFT ASEEPPCIIA
VLCELHDGLL VEAKGIKEHY FKPYISKLFD RKILKGECLL DLSSFTDNSK
AVNKEYEEYV LTVGDFDERI FLGADAEEEI GTPRKFTRDT PLGKLTAQAN
VEYNLQQHFE KKRSFAPSTP LTGRRYLREK EAVITPVASA TQSVSRLQSI
VAGLKNAPSD QLINIFESCV RNPVENIMKI LKGIGETFCQ HYTQSTDEQP
GSHIDFAVNR LKLAEILYYK ILETVMVQET RRLHGMDMSV LLEQDIFHRS
LMACCLEIVL FAYSSPRTFP WIIEVLNLQP FYFYKVIEVV IRSEEGLSRD
MVKHLNSIEE QILESLAWSH DSALWEALQV SANKVPTCEE VIFPNNFETG
NGGNVQGHLP LMPMSPLMHP RVKEVRTDSG SLRRDMQPLS PISVHERYSS
PTAGSAKRRL FGEDPPKEML MDKIITEGTK LKIAPSSSIT AENVSILPGQ
TLLTMATAPV TGTTGHKVTI PLHGVANDAG EITLIPLSMN TNQESKVKSP
VSLTAHSLIG ASPKQTNLTK AQEVHSTGIN RPKRTGSLAL FYRKVYHLAS
VRLRDLCLKL DVSNELRRKI WTCFEFTLVH CPDLMKDRHL DQLLLCAFYI
MAKVTKEERT FQEIMKSYRN QPQANSHVYR SVLLKSIPRE VVAYNKNIND
DFEMIDCDLE DATKTPDCSS GPVKEERGDL IKFYNTIYVG RVKSFALKYD
LANQDHMMDA PPLSPFPHIK QQPGSPRRIS QQHSIYISPH KNGSGLTPRS
ALLYKFNGSP SKSLKDINNM IRQGEQRTKK RVIAIDSDAE SPAKRVCQEN
DDVLLKRLQD VVSERANH
UCSC uc002xgi.5; human. [P28749-1];

Category

Human RBL1 ELISA Kit

Human 107 kDa retinoblastoma-associated protein ELISA Kit;Human p107 ELISA Kit;Human PRB1 ELISA Kit;Human CP107 ELISA Kit;Human RB transcriptional corepressor like 1 ELISA Kit;Human retinoblastoma-like protein 1 ELISA Kit;Human retinoblastoma-like 1 ELISA Kit;