Human RPA1 ELISA Kit

Alternative name
Human RP-A p70 ELISA Kit;Human replication factor A protein 1 ELISA Kit;Human RF-A protein 1 ELISA Kit;Human single-stranded DNA-binding protein ELISA Kit;Human REPA1 ELISA Kit;Human RPA70 ELISA Kit;Human HSSB ELISA Kit;Human MST075 ELISA Kit;Human RF-A ELISA Kit;Human RP-A ELISA Kit;Human replication protein A1 ELISA Kit;Human replication protein A 70 kDa DNA-binding subunit ELISA Kit;Human MSTP075 ELISA Kit;Human replication protein A1, 70kDa ELISA Kit;
Catalog
E033213
species
Human
GeneRPA1
Standard Curve
Other Species Mouse Rpa1 ELISA Kit Chicken RPA1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of RPA1. No significant cross-reactivity or interference between RPA1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human RPA1 is typically less than 0.106ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addi
Intended UseHuman RPA1 ELISA Kit allows for the in vitro quantitative determination of RPA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RPA1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RPA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RPA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RPA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Replication protein A 70 kDa DNA-binding subunit, N-terminally processed Protein information
Uniprot ID RFA1_HUMAN
Uniprot AC P27694; A8K0Y9; Q59ES9;
UniGene Hs.461925;
GeneID 6117
KEGG hsa:6117;
Human Replication protein A 70 kDa DNA-binding subunit, N-terminally processed Protein SEQUENCE
SEQUENCE 616 AA; 68138 MW; FE038F40F5886CD1 CRC64;
MVGQLSEGAI AAIMQKGDTN IKPILQVINI RPITTGNSPP RYRLLMSDGL
NTLSSFMLAT QLNPLVEEEQ LSSNCVCQIH RFIVNTLKDG RRVVILMELE
VLKSAEAVGV KIGNPVPYNE GLGQPQVAPP APAASPAASS RPQPQNGSSG
MGSTVSKAYG ASKTFGKAAG PSLSHTSGGT QSKVVPIASL TPYQSKWTIC
ARVTNKSQIR TWSNSRGEGK LFSLELVDES GEIRATAFNE QVDKFFPLIE
VNKVYYFSKG TLKIANKQFT AVKNDYEMTF NNETSVMPCE DDHHLPTVQF
DFTGIDDLEN KSKDSLVDII GICKSYEDAT KITVRSNNRE VAKRNIYLMD
TSGKVVTATL WGEDADKFDG SRQPVLAIKG ARVSDFGGRS LSVLSSSTII
ANPDIPEAYK LRGWFDAEGQ ALDGVSISDL KSGGVGGSNT NWKTLYEVKS
ENLGQGDKPD YFSSVATVVY LRKENCMYQA CPTQDCNKKV IDQQNGLYRC
EKCDTEFPNF KYRMILSVNI ADFQENQWVT CFQESAEAIL GQNAAYLGEL
KDKNEQAFEE VFQNANFRSF IFRVRVKVET YNDESRIKAT VMDVKPVDYR
EYGRRLVMSI RRSALM
UCSC uc002fto.3; human.;

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Human RPA1 ELISA Kit