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  • Alternative name

    MAPK/MAK/MRK overlapping kinase ELISA KIT; MOK protein kinase ELISA KIT; Renal tumor antigen 1 ELISA KIT; RAGE-1MOK ELISA KIT; RAGE ELISA KIT; RAGE1 ELISA KIT; RAGE-1 ELISA KIT

  • Catalog
  • species
  • GeneRAGE
  • Standard CurveHuman RAGE ELISA Kit
  • Other Species Human RAGE/AGER ELISA KitMouse RAGE/AGER ELISA KitMouse RAGE ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of RAGE. No significant cross-reactivity or interference between RAGE and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human RAGE is typically less than 0.066ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addi
  • Intended UseHuman RAGE ELISA Kit allows for the in vitro quantitative determination of RAGE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAGE. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RAGE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RAGE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RAGE in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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