Human RGS4 ELISA Kit

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  • Alternative name

    Human RGP4 ELISA Kit;Human RGS4 ELISA Kit;Human SCZD9 ELISA Kit;Human regulator of G-protein signaling 4 ELISA Kit;Human schizophrenia disorder 9 ELISA Kit;

  • Catalog
    E033109
  • species
    Human
  • GeneRGS4
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman RGS4 ELISA Kit allows for the in vitro quantitative determination of RGS4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the Assay: RGS4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for RGS4. Standards or samples are then added to the microtiter plate wells and RGS4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of RGS4 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for RGS4 are added to each well to "sandwich" the RGS4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain RGS4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RGS4 concentration in each sample is interpolated from this standard curve.
  • Human Regulator of G-protein signaling 4 Protein information
  • Uniprot ID RGS4_HUMAN
  • Uniprot AC P49798; A7XA56; A7XA58; A7XA59; A7YVV7; B1APZ3;
  • UniGene Hs.386726;
  • GeneID 5999
  • KEGG hsa:5999;
  • Human Regulator of G-protein signaling 4 Protein SEQUENCE
  • SEQUENCE 205 AA; 23256 MW; 7713F1F7496A698B CRC64;

    MCKGLAGLPA SCLRSAKDMK HRLGFLLQKS DSCEHNSSHN KKDKVVICQR

    VSQEEVKKWA ESLENLISHE CGLAAFKAFL KSEYSEENID FWISCEEYKK

    IKSPSKLSPK AKKIYNEFIS VQATKEVNLD SCTREETSRN MLEPTITCFD

    EAQKKIFNLM EKDSYRRFLK SRFYLDLVNP SSCGAEKQKG AKSSADCASL

    VPQCA

  • UCSC uc001gcl.5; human. [P49798-1];



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