Human RGN ELISA Kit

Alternative name
Human RC ELISA Kit;Human gluconolactonase ELISA Kit;Human GNL ELISA Kit;Human senescence marker protein 30 ELISA Kit;Human SMP-30 ELISA Kit;Human SMP30 ELISA Kit;Human HEL-S-41 ELISA Kit;Human regucalcin ELISA Kit;Human epididymis secretory protein Li 41 ELISA Kit;
Catalog
E033085
species
Human
GeneRGN
SpecificityThis assay has high sensitivity and excellent specificity for detection of Regucalcin (RGN). No significant cross-reactivity or interference between Regucalcin (RGN) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.109ng/mL.
Intended UseHuman RGN ELISA Kit allows for the in vitro quantitative determination of RGN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regucalcin (RGN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regucalcin (RGN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regucalcin (RGN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Regucalcin (RGN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Regucalcin Protein information
Uniprot ID RGN_HUMAN
Uniprot AC Q15493; A4FTW1; A8K271; Q53FC9; Q5JRR5;
UniGene Hs.77854;
GeneID 9104
KEGG hsa:9104;
Human Regucalcin Protein SEQUENCE
SEQUENCE 299 AA; 33253 MW; 95BA1C73B7B77635 CRC64;
MSSIKIECVL PENCRCGESP VWEEVSNSLL FVDIPAKKVC RWDSFTKQVQ
RVTMDAPVSS VALRQSGGYV ATIGTKFCAL NWKEQSAVVL ATVDNDKKNN
RFNDGKVDPA GRYFAGTMAE ETAPAVLERH QGALYSLFPD HHVKKYFDQV
DISNGLDWSL DHKIFYYIDS LSYSVDAFDY DLQTGQISNR RSVYKLEKEE
QIPDGMCIDA EGKLWVACYN GGRVIRLDPV TGKRLQTVKL PVDKTTSCCF
GGKNYSEMYV TCARDGMDPE GLLRQPEAGG IFKITGLGVK GIAPYSYAG
UCSC uc010nhp.2; human. [Q15493-1];

Category

Human RGN ELISA Kit

Human RC ELISA Kit;Human gluconolactonase ELISA Kit;Human GNL ELISA Kit;Human senescence marker protein 30 ELISA Kit;Human SMP-30 ELISA Kit;Human SMP30 ELISA Kit;Human HEL-S-41 ELISA Kit;Human regucalcin ELISA Kit;Human epididymis secretory protein Li 41 ELISA Kit;