Human REG3alpha ELISA Kit

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  • Alternative name

    Regenerating islet-derived protein 3-alpha ELISA KIT; Hepatointestinal pancreatic protein ELISA KIT; HIP/PAPREG3A ELISA KIT; HIP ELISA KIT; PAP ELISA KIT; PAP1 ELISA KIT; REG-3-alpha ELISA KIT; HIP/PAP ELISA KIT; Reg III-alpha ELISA KIT

  • Catalog
  • species
  • GeneREG3alpha
  • Other Species Mouse REG3alpha ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 9.4pg/ml
  • Intended UseHuman REG3alpha ELISA Kit allows for the in vitro quantitative determination of REG3alpha , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human REG3alpha antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human REG3 alpha antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human REG3alpha amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human REG3alpha can be calculated.

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