Human REG4 ELISA Kit

Alternative name
Human REG-4 ELISA Kit;Human gastrointestinal secretory protein ELISA Kit;Human REG-like protein ELISA Kit;Human regenerating islet-derived protein IV ELISA Kit;Human reg IV ELISA Kit;Human GISP ELISA Kit;Human RELP ELISA Kit;Human REG-IV ELISA Kit;Human regenerating family member 4 ELISA Kit;Human regenerating islet-derived protein 4 ELISA Kit;Human regenerating gene type IV ELISA Kit;Human regenerating islet-derived family, member 4 ELISA Kit;
Catalog
E033053
species
Human
GeneREG4
SpecificityNatural and recombinant human REG-4
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman REG4 ELISA Kit allows for the in vitro quantitative determination of REG4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human REG-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for REG-4 has been precoated onto 96-well plates. Standards(E.coli, D23-P158) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for REG-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human REG-4 amount of sample captured in plate. Background: Regenerating islet-derived protein 4 is a protein that in humans is encoded by the REG4 gene. It is mapped to 1p12. REG-4 gene contains 6 exons and that the exon structure is preserved among members of the REG gene family. This gene is able to maintain carbohydrate recognition activity in an acidic environment. REG-4 expression was significantly upregulated by inflammation and tissue injury associated with active Crohn disease and ulcerative colitis, and most of the colorectal tumors overexpressing REG-4 are mucinous tumors or neuroendocrine tumors.
Human Regenerating islet-derived protein 4 Protein information
Uniprot ID REG4_HUMAN
Uniprot AC Q9BYZ8; Q8NER6; Q8NER7;
UniGene Hs.660883;
GeneID 83998
KEGG hsa:83998;
Human Regenerating islet-derived protein 4 Protein SEQUENCE
SEQUENCE 158 AA; 18230 MW; 7308849CBBD6E93E CRC64;
MASRSMRLLL LLSCLAKTGV LGDIIMRPSC APGWFYHKSN CYGYFRKLRN
WSDAELECQS YGNGAHLASI LSLKEASTIA EYISGYQRSQ PIWIGLHDPQ
KRQQWQWIDG AMYLYRSWSG KSMGGNKHCA EMSSNNNFLT WSSNECNKRQ
HFLCKYRP
UCSC uc001eif.4; human. [Q9BYZ8-1];

Human REG4 ELISA Kit

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