Human RIPK2 ELISA Kit

Alternative name
Human CARD-containing interleukin-1 beta-converting enzyme-associated kinase ELISA Kit;Human CARD-containing IL-1 beta ICE-kinase ELISA Kit;Human RIP-like-interacting CLARP kinase ELISA Kit;Human receptor-interacting protein 2 ELISA Kit;Human RIP-2 ELISA Kit;Human tyrosine-protein kinase RIPK2 ELISA Kit;Human CARDIAK ELISA Kit;Human RICK ELISA Kit;Human RIP2 ELISA Kit;Human CARD3 ELISA Kit;Human CCK ELISA Kit;Human GIG30 ELISA Kit;Human receptor interacting serine/threonine kinase 2 ELISA Kit;Human receptor-interacting serine/threonine-protein kinase 2 ELISA Kit;Human CARD-carrying kinase ELISA Kit;Human CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase ELISA Kit;Human growth-inhibiting gene 30 ELISA Kit;Human receptor-interacting protein (RIP)-like interacting caspase-like apoptosis regulatory protein (CLARP) kinase ELISA Kit;
Catalog
E033007
species
Human
GeneRIPK2
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman RIPK2 ELISA Kit allows for the in vitro quantitative determination of RIPK2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: RIPK2 ELIS A kit applies the competitive enzyme iminunoassay technique utilizing a monoclonal anti-RIPK2 antibody and an RIPK2-HRP conjugate. The assay sample and buffer are incubated together with RIPK2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RIPK2 concentration since RIPK2 from samples and RIPK2-HRP conjugate compete for the anti-RIPK2 antibody binding site. Since the number of sites is limited, as more sites are occupied by RIPK2 from the sample, fewer sites are left to bind RIPK2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RIPK2 concentration in each sample is interpolated from this standard curve.
Human Receptor-interacting serine/threonine-protein kinase 2 Protein information
Uniprot ID RIPK2_HUMAN
Uniprot AC O43353; B7Z748; Q6UWF0;
UniGene Hs.103755;
GeneID 8767
KEGG hsa:8767;
Human Receptor-interacting serine/threonine-protein kinase 2 Protein SEQUENCE
SEQUENCE 540 AA; 61195 MW; 575A692239505792 CRC64;
MNGEAICSAL PTIPYHKLAD LRYLSRGASG TVSSARHADW RVQVAVKHLH
IHTPLLDSER KDVLREAEIL HKARFSYILP ILGICNEPEF LGIVTEYMPN
GSLNELLHRK TEYPDVAWPL RFRILHEIAL GVNYLHNMTP PLLHHDLKTQ
NILLDNEFHV KIADFGLSKW RMMSLSQSRS SKSAPEGGTI IYMPPENYEP
GQKSRASIKH DIYSYAVITW EVLSRKQPFE DVTNPLQIMY SVSQGHRPVI
NEESLPYDIP HRARMISLIE SGWAQNPDER PSFLKCLIEL EPVLRTFEEI
TFLEAVIQLK KTKLQSVSSA IHLCDKKKME LSLNIPVNHG PQEESCGSSQ
LHENSGSPET SRSLPAPQDN DFLSRKAQDC YFMKLHHCPG NHSWDSTISG
SQRAAFCDHK TTPCSSAIIN PLSTAGNSER LQPGIAQQWI QSKREDIVNQ
MTEACLNQSL DALLSRDLIM KEDYELVSTK PTRTSKVRQL LDTTDIQGEE
FAKVIVQKLK DNKQMGLQPY PEILVVSRSP SLNLLQNKSM
UCSC uc003yee.4; human. [O43353-1];

Category

Human RIPK2 ELISA Kit