Human RIPK1 ELISA Kit

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  • Alternative name

    Human cell death protein RIP ELISA Kit;Human receptor-interacting protein 1 ELISA Kit;Human RIP-1 ELISA Kit;Human serine/threonine-protein kinase RIP ELISA Kit;Human RIP ELISA Kit;Human RIP1 ELISA Kit;Human receptor interacting serine/threonine kinase 1 ELISA Kit;Human receptor-interacting serine/threonine-protein kinase 1 ELISA Kit;Human receptor (TNFRSF)-interacting serine-threonine kinase 1 ELISA Kit;Human receptor-interacting protein kinase 1 ELISA Kit;

  • Catalog
    E033006
  • species
    Human
  • GeneRIPK1
  • Standard CurveHuman RIPK1 ELISA Kit
  • Other Species Mouse Ripk1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Receptor Interacting Serine Threonine Kinase 1 (RIPK1). No significant cross-reactivity or interference between Receptor Interacting Serine Threonine Kinase 1 (RIPK1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.057ng/mL.
  • Intended UseHuman RIPK1 ELISA Kit allows for the in vitro quantitative determination of RIPK1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor Interacting Serine Threonine Kinase 1 (RIPK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor Interacting Serine Threonine Kinase 1 (RIPK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor Interacting Serine Threonine Kinase 1 (RIPK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Receptor Interacting Serine Threonine Kinase 1 (RIPK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Receptor-interacting serine/threonine-protein kinase 1 Protein information
  • Uniprot ID RIPK1_HUMAN
  • Uniprot AC Q13546; A0AV89; B2RAG1; B4E3F9; Q13180; Q59H33;
  • UniGene Hs.519842;
  • GeneID 8737
  • KEGG hsa:8737;
  • Human Receptor-interacting serine/threonine-protein kinase 1 Protein SEQUENCE
  • SEQUENCE 671 AA; 75931 MW; 976E2428D525A9B2 CRC64;

    MQPDMSLNVI KMKSSDFLES AELDSGGFGK VSLCFHRTQG LMIMKTVYKG

    PNCIEHNEAL LEEAKMMNRL RHSRVVKLLG VIIEEGKYSL VMEYMEKGNL

    MHVLKAEMST PLSVKGRIIL EIIEGMCYLH GKGVIHKDLK PENILVDNDF

    HIKIADLGLA SFKMWSKLNN EEHNELREVD GTAKKNGGTL YYMAPEHLND

    VNAKPTEKSD VYSFAVVLWA IFANKEPYEN AICEQQLIMC IKSGNRPDVD

    DITEYCPREI ISLMKLCWEA NPEARPTFPG IEEKFRPFYL SQLEESVEED

    VKSLKKEYSN ENAVVKRMQS LQLDCVAVPS SRSNSATEQP GSLHSSQGLG

    MGPVEESWFA PSLEHPQEEN EPSLQSKLQD EANYHLYGSR MDRQTKQQPR

    QNVAYNREEE RRRRVSHDPF AQQRPYENFQ NTEGKGTAYS SAASHGNAVH

    QPSGLTSQPQ VLYQNNGLYS SHGFGTRPLD PGTAGPRVWY RPIPSHMPSL

    HNIPVPETNY LGNTPTMPFS SLPPTDESIK YTIYNSTGIQ IGAYNYMEIG

    GTSSSLLDST NTNFKEEPAA KYQAIFDNTT SLTDKHLDPI RENLGKHWKN

    CARKLGFTQS QIDEIDHDYE RDGLKEKVYQ MLQKWVMREG IKGATVGKLA

    QALHQCSRID LLSSLIYVSQ N

  • UCSC uc003mux.4; human. [Q13546-1];


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