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Human RBP4 ELISA Kit

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  • Alternative name

    Human plasma retinol-binding protein ELISA Kit;Human PRBP ELISA Kit;Human RBP ELISA Kit;Human MCOPCB10 ELISA Kit;Human RDCCAS ELISA Kit;Human retinol binding protein 4 ELISA Kit;Human retinol-binding protein 4 ELISA Kit;Human retinol binding protein 4, plasma ELISA Kit;Human retinol-binding protein 4, interstitial ELISA Kit;
  • Catalog
    E032942
  • species
    Human
  • GeneRBP4
  • SpecificityNatural and recombinant human RBP4
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 10pg/ml
  • Intended UseHuman RBP4 ELISA Kit allows for the in vitro quantitative determination of RBP4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: human RBP4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for RBP4 has been precoated onto 96-well plates. Standards(NSO, E19-L201) and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for RBP4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human RBP4 amount of sample captured in plate. Background: Retinol binding protein 4, plasma, also known as RBP4, belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It is protein that in humans is encoded by the RBP4 gene. RBP4 gene resides just centromeric of the cluster of CYP2C genes on 10q24. The mouse Rbp4 locus is closely linked and just proximal to the locus for phenobarbital-inducible cytochrome P450-2c (Cyp-2c) at the distal end of chromosome 19. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. The standard used in this kit is recombinant protein, with E19-L201 aa sequence, the molecular weight is 22kda.
  • Human Plasma retinol-binding protein(1-176) Protein information
  • Uniprot ID RET4_HUMAN
  • Uniprot AC P02753; D3DR38; O43478; O43479; Q5VY24; Q8WWA3; Q9P178;
  • UniGene Hs.50223;
  • GeneID 5950
  • KEGG hsa:5950;
  • Human Plasma retinol-binding protein(1-176) Protein SEQUENCE

  • SEQUENCE 201 AA; 23010 MW; 660C6DD8CC9B811A CRC64;

    MKWVWALLLL AALGSGRAER DCRVSSFRVK ENFDKARFSG TWYAMAKKDP

    EGLFLQDNIV AEFSVDETGQ MSATAKGRVR LLNNWDVCAD MVGTFTDTED

    PAKFKMKYWG VASFLQKGND DHWIVDTDYD TYAVQYSCRL LNLDGTCADS

    YSFVFSRDPN GLPPEAQKIV RQRQEELCLA RQYRLIVHNG YCDGRSERNL

    L

  • UCSC uc001kit.4; human.;



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