Human RAB8A ELISA Kit

Alternative name
Human oncogene c-mel ELISA Kit;Human MEL ELISA Kit;Human RAB8 ELISA Kit;Human RAB8A, member RAS oncogene family ELISA Kit;Human ras-related protein Rab-8A ELISA Kit;Human mel transforming oncogene (RAB8 homolog) ELISA Kit;Human mel transforming oncogene (derived from cell line NK14) ELISA Kit;Human mel transforming oncogene (derived from cell line NK14)- RAB8 homolog ELISA Kit;Human ras-associated protein RAB8 ELISA Kit;
Catalog
E032929
species
Human
GeneRAB8A
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094ng/ml
Intended UseHuman RAB8A ELISA Kit allows for the in vitro quantitative determination of RAB8A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-RAB8A antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-RAB8A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the RAB8A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of RAB8A can be calculated.
Human Ras-related protein Rab-8A Protein information
Uniprot ID RAB8A_HUMAN
Uniprot AC P61006; B4DEK7; P24407; Q6FHV5;
UniGene Hs.642874;
GeneID 4218
KEGG hsa:4218;
Human Ras-related protein Rab-8A Protein SEQUENCE
SEQUENCE 207 AA; 23668 MW; AA52DBF54A2CD056 CRC64;
MAKTYDYLFK LLLIGDSGVG KTCVLFRFSE DAFNSTFIST IGIDFKIRTI
ELDGKRIKLQ IWDTAGQERF RTITTAYYRG AMGIMLVYDI TNEKSFDNIR
NWIRNIEEHA SADVEKMILG NKCDVNDKRQ VSKERGEKLA LDYGIKFMET
SAKANINVEN AFFTLARDIK AKMDKKLEGN SPQGSNQGVK ITPDQQKRSS
FFRCVLL
UCSC uc002ndn.5; human. [P61006-1];

Human RAB8A ELISA Kit

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