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Human RHOA ELISA Kit

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  • Alternative name

    Human Rho cDNA clone 12 ELISA Kit;Human h12 ELISA Kit;Human ARH12 ELISA Kit;Human ARHA ELISA Kit;Human RHO12 ELISA Kit;Human RHOH12 ELISA Kit;Human ras homolog family member A ELISA Kit;Human transforming protein RhoA ELISA Kit;Human Aplysia ras-related homolog 12 ELISA Kit;Human oncogene RHO H12 ELISA Kit;Human small GTP binding protein RhoA ELISA Kit;
  • Catalog
    E032864
  • species
    Human
  • GeneRHOA
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of RHOA. No significant cross-reactivity or interference between RHOA and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of RHOA is typically less than 0.057ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two
  • Intended UseHuman RHOA ELISA Kit allows for the in vitro quantitative determination of RHOA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RHOA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RHOA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RHOA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RHOA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Transforming protein RhoA Protein information
  • Uniprot ID RHOA_HUMAN
  • Uniprot AC P61586; P06749; Q53HM4; Q5U024; Q9UDJ0; Q9UEJ4;
  • UniGene Hs.247077;
  • GeneID 387
  • KEGG hsa:387;
  • Human Transforming protein RhoA Protein SEQUENCE

  • SEQUENCE 193 AA; 21768 MW; C4DA2DC31FF858BC CRC64;

    MAAIRKKLVI VGDGACGKTC LLIVFSKDQF PEVYVPTVFE NYVADIEVDG

    KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSIDSPD SLENIPEKWT

    PEVKHFCPNV PIILVGNKKD LRNDEHTRRE LAKMKQEPVK PEEGRDMANR

    IGAFGYMECS AKTKDGVREV FEMATRAALQ ARRGKKKSGC LVL

  • UCSC uc003cwu.4; human.;



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