Human RAMP1 ELISA Kit

Alternative name
Human Calcitonin-receptor-like receptor activity-modifying protein 1 ELISA Kit;Human CRLR activity-modifying protein 1 ELISA Kit;Human receptor activity modifying protein 1 ELISA Kit;Human receptor activity-modifying protein 1 ELISA Kit;Human calcitonin receptor-like receptor activity modifying protein 1 ELISA Kit;Human receptor (G protein-coupled) activity modifying protein 1 ELISA Kit;Human receptor (calcitonin) activity modifying protein 1 ELISA Kit;
Catalog
E032788
species
Human
GeneRAMP1
SpecificityThis assay has high sensitivity and excellent specificity for detection of RAMP1 . No significant cross-reactivity or interference between RAMP1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 14.063pg/ml
Intended UseHuman RAMP1 ELISA Kit allows for the in vitro quantitative determination of RAMP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- RAMP1antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- RAMP1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the RAMP1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of RAMP1 can be calculated.
Human Receptor activity-modifying protein 1 Protein information
Uniprot ID RAMP1_HUMAN
Uniprot AC O60894; Q6FGS5;
UniGene Hs.471783;
GeneID 10267
KEGG hsa:10267;
Human Receptor activity-modifying protein 1 Protein SEQUENCE
SEQUENCE 148 AA; 16988 MW; 8530DD590BAEBE5C CRC64;
MARALCRLPR RGLWLLLAHH LFMTTACQEA NYGALLRELC LTQFQVDMEA
VGETLWCDWG RTIRSYRELA DCTWHMAEKL GCFWPNAEVD RFFLAVHGRY
FRSCPISGRA VRDPPGSILY PFIVVPITVT LLVTALVVWQ SKRTEGIV
UCSC uc002vxj.4; human.;

Human RAMP1 ELISA Kit

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