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Human RAD51 ELISA Kit

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  • Alternative name

    Human HsRad51 ELISA Kit;Human HRAD51 ELISA Kit;Human RAD51 homolog A ELISA Kit;Human RAD51A ELISA Kit;Human RECA ELISA Kit;Human BRCC5 ELISA Kit;Human FANCR ELISA Kit;Human HsT16930 ELISA Kit;Human MRMV2 ELISA Kit;Human RAD51 recombinase ELISA Kit;Human DNA repair protein RAD51 homolog 1 ELISA Kit;Human BRCA1/BRCA2-containing complex, subunit 5 ELISA Kit;Human RecA, E. coli, homolog of ELISA Kit;Human RecA-like protein ELISA Kit;Human recombination protein A ELISA Kit;
  • Catalog
    E032745
  • species
    Human
  • GeneRAD51
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of RAD51 Homolog (RAD51). No significant cross-reactivity or interference between RAD51 Homolog (RAD51) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.118ng/mL.
  • Intended UseHuman RAD51 ELISA Kit allows for the in vitro quantitative determination of RAD51 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAD51 Homolog (RAD51). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RAD51 Homolog (RAD51). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RAD51 Homolog (RAD51), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RAD51 Homolog (RAD51) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human DNA repair protein RAD51 homolog 1 Protein information
  • Uniprot ID RAD51_HUMAN
  • Uniprot AC Q06609; B0FXP0; B2R8T6; Q6FHX9; Q6ZNA8; Q9BV60;
  • UniGene Hs.631709;
  • GeneID 5888
  • KEGG hsa:5888;
  • Human DNA repair protein RAD51 homolog 1 Protein SEQUENCE

  • SEQUENCE 339 AA; 36966 MW; 26578E6206DEDEDA CRC64;

    MAMQMQLEAN ADTSVEEESF GPQPISRLEQ CGINANDVKK LEEAGFHTVE

    AVAYAPKKEL INIKGISEAK ADKILAEAAK LVPMGFTTAT EFHQRRSEII

    QITTGSKELD KLLQGGIETG SITEMFGEFR TGKTQICHTL AVTCQLPIDR

    GGGEGKAMYI DTEGTFRPER LLAVAERYGL SGSDVLDNVA YARAFNTDHQ

    TQLLYQASAM MVESRYALLI VDSATALYRT DYSGRGELSA RQMHLARFLR

    MLLRLADEFG VAVVITNQVV AQVDGAAMFA ADPKKPIGGN IIAHASTTRL

    YLRKGRGETR ICKIYDSPCL PEAEAMFAIN ADGVGDAKD

  • UCSC uc001zmi.5; human. [Q06609-1];



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