Human RSPO4 ELISA Kit

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  • Alternative name

    Human roof plate-specific spondin-4 ELISA Kit;Human hRspo4 ELISA Kit;Human C20orf182 ELISA Kit;Human CRISTIN4 ELISA Kit;Human R-spondin 4 ELISA Kit;Human R-spondin-4 ELISA Kit;Human R-spondin family, member 4 ELISA Kit;

  • Catalog
    E032623
  • species
    Human
  • GeneRSPO4
  • Standard CurveHuman RSPO4 ELISA Kit
  • Other Species Mouse Rspo4 ELISA Kit
  • SpecificityNatural and recombinant Human R-Spondin-4
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Intra-assay Precision Intra-Assay Precision (Precision within an assay) Three samples of kn
  • Intended UseHuman RSPO4 ELISA Kit allows for the in vitro quantitative determination of RSPO4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: Human R-Spondin-4 ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for R-Spondin-4 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: L20-P234) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for R-Spondin-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human R-Spondin-4 amount of sample captured in plate. Background/Introduction: R-spondin 4 is a protein in humans that is encoded by the RSPO4 gene. It is mapped to chromosome 20p13. This gene encodes a member of the R-spondin family of proteins that share a common domain organization consisting of a signal peptide, cysteine-rich/furin-like domain, thrombospondin domain and a C-terminal basic region. The encoded protein may be involved in activation of Wnt/beta-catenin signaling pathways. Mutations in this gene are associated with anonychia congenital. Alternate splicing results in multiple transcript variants.
  • Human R-spondin-4 Protein information
  • Uniprot ID RSPO4_HUMAN
  • Uniprot AC Q2I0M5; A2A2I6; Q9UGB2;
  • UniGene Hs.444980;
  • GeneID 343637
  • KEGG hsa:343637;
  • Human R-spondin-4 Protein SEQUENCE
  • SEQUENCE 234 AA; 26171 MW; 853E4494533B73F7 CRC64;

    MRAPLCLLLL VAHAVDMLAL NRRKKQVGTG LGGNCTGCII CSEENGCSTC

    QQRLFLFIRR EGIRQYGKCL HDCPPGYFGI RGQEVNRCKK CGATCESCFS

    QDFCIRCKRQ FYLYKGKCLP TCPPGTLAHQ NTRECQGECE LGPWGGWSPC

    THNGKTCGSA WGLESRVREA GRAGHEEAAT CQVLSESRKC PIQRPCPGER

    SPGQKKGRKD RRPRKDRKLD RRLDVRPRQP GLQP

  • UCSC uc002wej.4; human. [Q2I0M5-1];




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