Human RSPO3 ELISA Kit

Alternative name
Human protein with TSP type-1 repeat ELISA Kit;Human hPWTSR ELISA Kit;Human roof plate-specific spondin-3 ELISA Kit;Human hRspo3 ELISA Kit;Human thrombospondin type-1 domain-containing protein 2 ELISA Kit;Human PWTSR ELISA Kit;Human THSD2 ELISA Kit;Human CRISTIN1 ELISA Kit;Human R-spondin 3 ELISA Kit;Human R-spondin-3 ELISA Kit;Human R-spondin 3 homolog ELISA Kit;Human thrombospondin, type I, domain containing 2 ELISA Kit;
Catalog
E032616
species
Human
GeneRSPO3
SpecificityThis assay has high sensitivity and excellent specificity for detection of R-Spondin 3 (RSPO3). No significant cross-reactivity or interference between R-Spondin 3 (RSPO3) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.118ng/mL.
Intended UseHuman RSPO3 ELISA Kit allows for the in vitro quantitative determination of RSPO3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to R-Spondin 3 (RSPO3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to R-Spondin 3 (RSPO3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain R-Spondin 3 (RSPO3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of R-Spondin 3 (RSPO3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human R-spondin-3 Protein information
Uniprot ID RSPO3_HUMAN
Uniprot AC Q9BXY4; B2RC27; Q5VTV4; Q96K87;
UniGene Hs.135254;
GeneID 84870
KEGG hsa:84870;
Human R-spondin-3 Protein SEQUENCE
SEQUENCE 272 AA; 30929 MW; CACAEC6B7E781189 CRC64;
MHLRLISWLF IILNFMEYIG SQNASRGRRQ RRMHPNVSQG CQGGCATCSD
YNGCLSCKPR LFFALERIGM KQIGVCLSSC PSGYYGTRYP DINKCTKCKA
DCDTCFNKNF CTKCKSGFYL HLGKCLDNCP EGLEANNHTM ECVSIVHCEV
SEWNPWSPCT KKGKTCGFKR GTETRVREII QHPSAKGNLC PPTNETRKCT
VQRKKCQKGE RGKKGRERKR KKPNKGESKE AIPDSKSLES SKEIPEQREN
KQQQKKRKVQ DKQKSVSVST VH
UCSC uc003qar.5; human. [Q9BXY4-1];

Human RSPO3 ELISA Kit

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