Human RSPO1 ELISA Kit

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  • Alternative name

    Human roof plate-specific spondin-1 ELISA Kit;Human hRspo1 ELISA Kit;Human CRISTIN3 ELISA Kit;Human RSPO ELISA Kit;Human R-spondin 1 ELISA Kit;Human R-spondin-1 ELISA Kit;Human R-spondin homolog ELISA Kit;

  • Catalog
    E032611
  • species
    Human
  • GeneRSPO1
  • Standard CurveHuman RSPO1 ELISA Kit
  • Other Species Mouse Rspo1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of RSPO1. No significant cross-reactivity or interference between RSPO1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RSPO1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman RSPO1 ELISA Kit allows for the in vitro quantitative determination of RSPO1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: RSPO1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RSPO1 antibody and an RSPO1-HRP conjugate. The assay sample and buffer are incubated together with RSPO1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RSPO1 concentration since RSPO1 from samples and RSPO1-HRP conjugate compete for the anti-RSPO1 antibody binding site. Since the number of sites is limited, as more sites are occupied by RSPO1 from the sample, fewer sites are left to bind RSPO1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RSPO1 concentration in each sample is interpolated from this standard curve.
  • Human R-spondin-1 Protein information
  • Uniprot ID RSPO1_HUMAN
  • Uniprot AC Q2MKA7; A2A420; Q0H8S6; Q14C72; Q5T0F2; Q8N7L5;
  • UniGene Hs.135015;
  • GeneID 284654
  • KEGG hsa:284654;
  • Human R-spondin-1 Protein SEQUENCE
  • SEQUENCE 263 AA; 28959 MW; 43794A881B1C0278 CRC64;

    MRLGLCVVAL VLSWTHLTIS SRGIKGKRQR RISAEGSQAC AKGCELCSEV

    NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE

    HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM

    SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT

    VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ

    QGTVGPLTSA GPA

  • UCSC uc031txm.2; human. [Q2MKA7-1];



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