Human PDHA2 ELISA Kit

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  • Alternative name

    Human PDHE1-A type II ELISA Kit;Human PDHAL ELISA Kit;Human pyruvate dehydrogenase (lipoamide) alpha 2 ELISA Kit;Human pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial ELISA Kit;Human pyruvate dehydrogenase, E1-alpha polypeptide, testis specific ELISA Kit;

  • Catalog
    E032570
  • species
    Human
  • GenePDHA2
  • Other Species Mouse Pdha2 ELISA KitRat Pdha2 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.094ng/ml
  • Intended UseHuman PDHA2 ELISA Kit allows for the in vitro quantitative determination of PDHA2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human PDHA2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PDHA2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PDHA2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PDHA2 can be calculated.
  • Human Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial Protein information
  • Uniprot ID ODPAT_HUMAN
  • Uniprot AC P29803; B2R9Q3; Q0VDI5; Q4VC02; Q6NXQ1;
  • UniGene Hs.131361;
  • GeneID 5161
  • KEGG hsa:5161;
  • Human Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial Protein SEQUENCE
  • SEQUENCE 388 AA; 42933 MW; 075B6CFF6DC73CC5 CRC64;

    MLAAFISRVL RRVAQKSARR VLVASRNSSN DATFEIKKCD LYLLEEGPPV

    TTVLTRAEGL KYYRMMLTVR RMELKADQLY KQKFIRGFCH LCDGQEACCV

    GLEAGINPSD HVITSYRAHG VCYTRGLSVR SILAELTGRR GGCAKGKGGS

    MHMYTKNFYG GNGIVGAQGP LGAGIALACK YKGNDEICLT LYGDGAANQG

    QIAEAFNMAA LWKLPCVFIC ENNLYGMGTS TERAAASPDY YKRGNFIPGL

    KVDGMDVLCV REATKFAANY CRSGKGPILM ELQTYRYHGH SMSDPGVSYR

    TREEIQEVRS KRDPIIILQD RMVNSKLATV EELKEIGAEV RKEIDDAAQF

    ATTDPEPHLE ELGHHIYSSD SSFEVRGANP WIKFKSVS

  • UCSC uc003htr.5; human.;



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