Human APOE ELISA Kit

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  • Alternative name

    Human APO-E ELISA Kit;Human AD2 ELISA Kit;Human ApoE4 ELISA Kit;Human LDLCQ5 ELISA Kit;Human LPG ELISA Kit;Human apolipoprotein E ELISA Kit;Human apolipoprotein E3 ELISA Kit;

  • Catalog
    E003255
  • species
    Human
  • GeneAPOE
  • Standard CurveHuman APOE ELISA Kit
  • Other Species Mouse Apoe ELISA KitBovine APOE ELISA KitCanine APOE ELISA KitGuinea Pig APOE ELISA KitMonkey APOE ELISA KitPorcine APOE ELISA KitRabbit APOE ELISA KitRat Apoe ELISA KitSheep APOE ELISA Kit
  • SpecificityNatural human APOE
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Sample For quantitative detection of human APOE in cell culture supernates, serum, plasma(h
  • Intended UseHuman APOE ELISA Kit allows for the in vitro quantitative determination of APOE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: Human APOE ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for APOE has been precoated onto 96-well plates. Standards(Expression system for standard: E Coli, Immunogen sequence: K19-H317) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for APOE is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human APOE amount of sample captured in plate. Background: Apolipoprotein E (APOE) is a class of apolipoprotein found in the chylomicron and Intermediate-density lipoprotein (IDLs) that is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. In peripheral tissues, APOE is primarily produced by the liver and macrophages, and mediates cholesterol metabolism in an isoform- dependent manner. In the central nervous system, APOE is mainly produced by astrocytes, and transports cholesterol to neurons via APOE receptors, which are members of the low density lipoprotein receptor gene family. This protein is involved in Alzheimer's disease and cardiovascular disease.
  • Human Apolipoprotein E Protein information
  • Uniprot ID APOE_HUMAN
  • Uniprot AC P02649; B2RC15; C0JYY5; Q9P2S4;
  • UniGene Hs.654439;
  • GeneID 348
  • KEGG hsa:348;
  • Human Apolipoprotein E Protein SEQUENCE
  • SEQUENCE 317 AA; 36154 MW; 91AFC04210A30689 CRC64;

    MKVLWAALLV TFLAGCQAKV EQAVETEPEP ELRQQTEWQS GQRWELALGR

    FWDYLRWVQT LSEQVQEELL SSQVTQELRA LMDETMKELK AYKSELEEQL

    TPVAEETRAR LSKELQAAQA RLGADMEDVC GRLVQYRGEV QAMLGQSTEE

    LRVRLASHLR KLRKRLLRDA DDLQKRLAVY QAGAREGAER GLSAIRERLG

    PLVEQGRVRA ATVGSLAGQP LQERAQAWGE RLRARMEEMG SRTRDRLDEV

    KEQVAEVRAK LEEQAQQIRL QAEAFQARLK SWFEPLVEDM QRQWAGLVEK

    VQAAVGTSAA PVPSDNH

  • UCSC uc002pab.4; human.;


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