Human PYGB ELISA Kit

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  • Alternative name

    Human GPBB ELISA Kit;Human glycogen phosphorylase B ELISA Kit;Human glycogen phosphorylase, brain form ELISA Kit;Human phosphorylase, glycogen; brain ELISA Kit;

  • Catalog
    E032518
  • species
    Human
  • GenePYGB
  • Other Species Mouse Pygb ELISA KitBovine PYGB ELISA KitRat Pygb ELISA KitSheep PYGB ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human PYGB. No significant cross-reactivity or interference between Human PYGB and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human PYGB and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman PYGB ELISA Kit allows for the in vitro quantitative determination of PYGB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PYGB in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PYGB. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PYGB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PYGB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PYGB. You can calculate the concentration of Human PYGB in the samples by comparing the OD of the samples to the standard curve.
  • Human Glycogen phosphorylase, brain form Protein information
  • Uniprot ID PYGB_HUMAN
  • Uniprot AC P11216; Q96AK1; Q9NPX8;
  • UniGene Hs.368157;
  • GeneID 5834
  • KEGG hsa:5834;
  • Human Glycogen phosphorylase, brain form Protein SEQUENCE
  • SEQUENCE 843 AA; 96696 MW; 810BFAD3002CACB0 CRC64;

    MAKPLTDSEK RKQISVRGLA GLGDVAEVRK SFNRHLHFTL VKDRNVATPR

    DYFFALAHTV RDHLVGRWIR TQQHYYERDP KRIYYLSLEF YMGRTLQNTM

    VNLGLQNACD EAIYQLGLDL EELEEIEEDA GLGNGGLGRL AACFLDSMAT

    LGLAAYGYGI RYEFGIFNQK IVNGWQVEEA DDWLRYGNPW EKARPEYMLP

    VHFYGRVEHT PDGVKWLDTQ VVLAMPYDTP VPGYKNNTVN TMRLWSAKAP

    NDFKLQDFNV GDYIEAVLDR NLAENISRVL YPNDNFFEGK ELRLKQEYFV

    VAATLQDIIR RFKSSKFGCR DPVRTCFETF PDKVAIQLND THPALSIPEL

    MRILVDVEKV DWDKAWEITK KTCAYTNHTV LPEALERWPV SMFEKLLPRH

    LEIIYAINQR HLDHVAALFP GDVDRLRRMS VIEEGDCKRI NMAHLCVIGS

    HAVNGVARIH SEIVKQSVFK DFYELEPEKF QNKTNGITPR RWLLLCNPGL

    ADTIVEKIGE EFLTDLSQLK KLLPLVSDEV FIRDVAKVKQ ENKLKFSAFL

    EKEYKVKINP SSMFDVHVKR IHEYKRQLLN CLHVVTLYNR IKRDPAKAFV

    PRTVMIGGKA APGYHMAKLI IKLVTSIGDV VNHDPVVGDR LKVIFLENYR

    VSLAEKVIPA ADLSQQISTA GTEASGTGNM KFMLNGALTI GTMDGANVEM

    AEEAGAENLF IFGLRVEDVE ALDRKGYNAR EYYDHLPELK QAVDQISSGF

    FSPKEPDCFK DIVNMLMHHD RFKVFADYEA YMQCQAQVDQ LYRNPKEWTK

    KVIRNIACSG KFSSDRTITE YAREIWGVEP SDLQIPPPNI PRD

  • UCSC uc002wup.4; human.;


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