-
Alternative name
Human Pro-MCH variant ELISA Kit;Human Pro-melanin-concentrating hormone-like protein 1 ELISA Kit;
-
Catalog
E032473
-
species
Human
-
GenePMCHL1
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
Intended UseHuman PMCHL1 ELISA Kit allows for the in vitro quantitative determination of PMCHL1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageStore the whole ELISA kit at 4℃
-
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human PMCHL1antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PMCHL1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PMCHL1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PMCHL1can be calculated.
-
Human Putative pro-MCH-like protein 1 Protein information
-
Uniprot ID
MCHL1_HUMAN
-
Uniprot AC
Q16048; Q9BQ10;
-
UniGene
Hs.247975;
-
Human Putative pro-MCH-like protein 1 Protein SEQUENCE
-
SEQUENCE 86 AA; 9715 MW; BC5EC470EDA8F7F3 CRC64;
MLSQKPKKKH NFLNHGLSLN LVIKPYLALE GSVAFPAENG VQDTESTQEK
RETGDEENSA
KFPVGRRDFD TLSCMLGRVY QSCWQV
-