Human APOA1 ELISA Kit

Contact Us
  • Alternative name

    Human apo-AI ELISA Kit;Human ApoA-I ELISA Kit;Human apolipoprotein A1 ELISA Kit;Human ProapoA-I ELISA Kit;Human Apolipoprotein A-I(1-242) ELISA Kit;Human apo(a) ELISA Kit;Human apolipoprotein A-I ELISA Kit;

  • Catalog
    E003239
  • species
    Human
  • GeneAPOA1
  • Standard CurveHuman APOA1 ELISA Kit
  • Other Species Mouse Apoa1 ELISA KitMouse Anti-APOA1 ELISA KitBovine APOA1 ELISA KitCanine APOA1 ELISA KitGuinea Pig APOA1 ELISA KitChicken APOA1 ELISA KitMonkey APOA1 ELISA KitPorcine APOA1 ELISA KitRabbit APOA1 ELISA KitRat Apoa1 ELISA Kit
  • SpecificityNatural human APOA1
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Sample For quantitative detection of human APOA1 in cell culture supernates, serum, plasma(
  • Intended UseHuman APOA1 ELISA Kit allows for the in vitro quantitative determination of APOA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: Human APOA1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for APOA1 has been precoated onto 96-well plates. Standards(Expression system for standard: E Coli, mmunogen sequence: D25-Q267) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for APOA1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human APOA1 amount of sample captured in plate. Background: Apolipoprotein A1 is a protein that in humans is encoded by the APOA1 gene. This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein.
  • Human Truncated apolipoprotein A-I Protein information
  • Uniprot ID APOA1_HUMAN
  • Uniprot AC P02647; A8K866; Q6LDN9; Q6Q785; Q9UCS8; Q9UCT8;
  • UniGene Hs.93194;
  • GeneID 335
  • KEGG hsa:335;
  • Human Truncated apolipoprotein A-I Protein SEQUENCE
  • SEQUENCE 267 AA; 30778 MW; 1A28B8366E620310 CRC64;

    MKAAVLTLAV LFLTGSQARH FWQQDEPPQS PWDRVKDLAT VYVDVLKDSG

    RDYVSQFEGS ALGKQLNLKL LDNWDSVTST FSKLREQLGP VTQEFWDNLE

    KETEGLRQEM SKDLEEVKAK VQPYLDDFQK KWQEEMELYR QKVEPLRAEL

    QEGARQKLHE LQEKLSPLGE EMRDRARAHV DALRTHLAPY SDELRQRLAA

    RLEALKENGG ARLAEYHAKA TEHLSTLSEK AKPALEDLRQ GLLPVLESFK

    VSFLSALEEY TKKLNTQ

  • UCSC uc001ppv.2; human.;




tag

Inquiry About Human APOA1 ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!