Human APOA1 ELISA Kit

Alternative name
Human apo-AI ELISA Kit;Human ApoA-I ELISA Kit;Human apolipoprotein A1 ELISA Kit;Human ProapoA-I ELISA Kit;Human Apolipoprotein A-I(1-242) ELISA Kit;Human apo(a) ELISA Kit;Human apolipoprotein A-I ELISA Kit;
Catalog
E003239
species
Human
GeneAPOA1
Standard Curve
Other Species Mouse Apoa1 ELISA Kit Mouse Anti-APOA1 ELISA Kit Bovine APOA1 ELISA Kit Canine APOA1 ELISA Kit Guinea Pig APOA1 ELISA Kit Chicken APOA1 ELISA Kit Monkey APOA1 ELISA Kit Porcine APOA1 ELISA Kit Rabbit APOA1 ELISA Kit Rat Apoa1 ELISA Kit
SpecificityNatural human APOA1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity Sample For quantitative detection of human APOA1 in cell culture supernates, serum, plasma(
Intended UseHuman APOA1 ELISA Kit allows for the in vitro quantitative determination of APOA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: Human APOA1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for APOA1 has been precoated onto 96-well plates. Standards(Expression system for standard: E Coli, mmunogen sequence: D25-Q267) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for APOA1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human APOA1 amount of sample captured in plate. Background: Apolipoprotein A1 is a protein that in humans is encoded by the APOA1 gene. This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein.
Human Truncated apolipoprotein A-I Protein information
Uniprot ID APOA1_HUMAN
Uniprot AC P02647; A8K866; Q6LDN9; Q6Q785; Q9UCS8; Q9UCT8;
UniGene Hs.93194;
GeneID 335
KEGG hsa:335;
Human Truncated apolipoprotein A-I Protein SEQUENCE
SEQUENCE 267 AA; 30778 MW; 1A28B8366E620310 CRC64;
MKAAVLTLAV LFLTGSQARH FWQQDEPPQS PWDRVKDLAT VYVDVLKDSG
RDYVSQFEGS ALGKQLNLKL LDNWDSVTST FSKLREQLGP VTQEFWDNLE
KETEGLRQEM SKDLEEVKAK VQPYLDDFQK KWQEEMELYR QKVEPLRAEL
QEGARQKLHE LQEKLSPLGE EMRDRARAHV DALRTHLAPY SDELRQRLAA
RLEALKENGG ARLAEYHAKA TEHLSTLSEK AKPALEDLRQ GLLPVLESFK
VSFLSALEEY TKKLNTQ
UCSC uc001ppv.2; human.;

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Category

Human APOA1 ELISA Kit

Human apo-AI ELISA Kit;Human ApoA-I ELISA Kit;Human apolipoprotein A1 ELISA Kit;Human ProapoA-I ELISA Kit;Human Apolipoprotein A-I(1-242) ELISA Kit;Human apo(a) ELISA Kit;Human apolipoprotein A-I ELISA Kit;