Human NPEPPS ELISA Kit

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  • Alternative name

    Human PSA ELISA Kit;Human cytosol alanyl aminopeptidase ELISA Kit;Human AAP-S ELISA Kit;Human MP100 ELISA Kit;Human aminopeptidase puromycin sensitive ELISA Kit;Human puromycin-sensitive aminopeptidase ELISA Kit;Human metalloproteinase MP100 ELISA Kit;

  • Catalog
    E032349
  • species
    Human
  • GeneNPEPPS
  • Other Species Mouse Npepps ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.188ng/ml
  • Intended UseHuman NPEPPS ELISA Kit allows for the in vitro quantitative determination of NPEPPS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human NPEPPS antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human NPEPPS antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human NPEPPS amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human NPEPPS can be calculated.
  • Human Puromycin-sensitive aminopeptidase Protein information
  • Uniprot ID PSA_HUMAN
  • Uniprot AC P55786; B7Z463; Q6P145; Q9NP16; Q9UEM2;
  • UniGene Hs.443837;
  • GeneID 9520
  • KEGG hsa:9520;
  • Human Puromycin-sensitive aminopeptidase Protein SEQUENCE
  • SEQUENCE 919 AA; 103276 MW; 873C9BF75494A057 CRC64;

    MWLAAAAPSL ARRLLFLGPP PPPLLLLVFS RSSRRRLHSL GLAAMPEKRP

    FERLPADVSP INYSLCLKPD LLDFTFEGKL EAAAQVRQAT NQIVMNCADI

    DIITASYAPE GDEEIHATGF NYQNEDEKVT LSFPSTLQTG TGTLKIDFVG

    ELNDKMKGFY RSKYTTPSGE VRYAAVTQFE ATDARRAFPC WDEPAIKATF

    DISLVVPKDR VALSNMNVID RKPYPDDENL VEVKFARTPV MSTYLVAFVV

    GEYDFVETRS KDGVCVRVYT PVGKAEQGKF ALEVAAKTLP FYKDYFNVPY

    PLPKIDLIAI ADFAAGAMEN WGLVTYRETA LLIDPKNSCS SSRQWVALVV

    GHELAHQWFG NLVTMEWWTH LWLNEGFASW IEYLCVDHCF PEYDIWTQFV

    SADYTRAQEL DALDNSHPIE VSVGHPSEVD EIFDAISYSK GASVIRMLHD

    YIGDKDFKKG MNMYLTKFQQ KNAATEDLWE SLENASGKPI AAVMNTWTKQ

    MGFPLIYVEA EQVEDDRLLR LSQKKFCAGG SYVGEDCPQW MVPITISTSE

    DPNQAKLKIL MDKPEMNVVL KNVKPDQWVK LNLGTVGFYR TQYSSAMLES

    LLPGIRDLSL PPVDRLGLQN DLFSLARAGI ISTVEVLKVM EAFVNEPNYT

    VWSDLSCNLG ILSTLLSHTD FYEEIQEFVK DVFSPIGERL GWDPKPGEGH

    LDALLRGLVL GKLGKAGHKA TLEEARRRFK DHVEGKQILS ADLRSPVYLT

    VLKHGDGTTL DIMLKLHKQA DMQEEKNRIE RVLGATLLPD LIQKVLTFAL

    SEEVRPQDTV SVIGGVAGGS KHGRKAAWKF IKDNWEELYN RYQGGFLISR

    LIKLSVEGFA VDKMAGEVKA FFESHPAPSA ERTIQQCCEN ILLNAAWLKR

    DAESIHQYLL QRKASPPTV

  • UCSC uc002ilr.5; human. [P55786-1];



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