Human APEX1 ELISA Kit

Alternative name
Human APEX nuclease ELISA Kit;Human APEN ELISA Kit;Human apurinic-apyrimidinic endonuclease 1 ELISA Kit;Human AP endonuclease 1 ELISA Kit;Human APE-1 ELISA Kit;Human REF-1 ELISA Kit;Human redox factor-1 ELISA Kit;Human APE ELISA Kit;Human APE1 ELISA Kit;Human APEX ELISA Kit;Human APX ELISA Kit;Human HAP1 ELISA Kit;Human REF1 ELISA Kit;Human apurinic/apyrimidinic endodeoxyribonuclease 1 ELISA Kit;Human DNA-(apurinic or apyrimidinic site) lyase ELISA Kit;Human AP endonuclease class I ELISA Kit;Human AP lyase ELISA Kit;Human APEX nuclease (multifunctional DNA repair enzyme) 1 ELISA Kit;Human apurinic/apyrimidinic (abasic) endonuclease ELISA Kit;Human deoxyribonuclease (apurinic or apyrimidinic) ELISA Kit;Human protein REF-1 ELISA Kit;
Catalog
E003231
species
Human
GeneAPEX1
Other Species Mouse Apex1 ELISA Kit Bovine APEX1 ELISA Kit Rat Apex1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of human APEX1. No significant cross-reactivity or interference between human APEX1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity3.9 pg/ml.
Intended UseHuman APEX1 ELISA Kit allows for the in vitro quantitative determination of APEX1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for APEX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any APEX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APEX1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APEX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human DNA-(apurinic or apyrimidinic site) lyase, mitochondrial Protein information
Uniprot ID APEX1_HUMAN
Uniprot AC P27695; Q969L5; Q99775;
UniGene Hs.73722;
GeneID 328
KEGG hsa:328;
Human DNA-(apurinic or apyrimidinic site) lyase, mitochondrial Protein SEQUENCE
SEQUENCE 318 AA; 35555 MW; B88579C01BAF80C6 CRC64;
MPKRGKKGAV AEDGDELRTE PEAKKSKTAA KKNDKEAAGE GPALYEDPPD
QKTSPSGKPA TLKICSWNVD GLRAWIKKKG LDWVKEEAPD ILCLQETKCS
ENKLPAELQE LPGLSHQYWS APSDKEGYSG VGLLSRQCPL KVSYGIGDEE
HDQEGRVIVA EFDSFVLVTA YVPNAGRGLV RLEYRQRWDE AFRKFLKGLA
SRKPLVLCGD LNVAHEEIDL RNPKGNKKNA GFTPQERQGF GELLQAVPLA
DSFRHLYPNT PYAYTFWTYM MNARSKNVGW RLDYFLLSHS LLPALCDSKI
RSKALGSDHC PITLYLAL
UCSC uc058yte.1; human.;

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Human APEX1 ELISA Kit