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Human PTPRK ELISA Kit

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  • Alternative name

    Human protein-tyrosine phosphatase kappa ELISA Kit;Human R-PTP-kappa ELISA Kit;Human PTPK ELISA Kit;Human protein tyrosine phosphatase, receptor type K ELISA Kit;Human receptor-type tyrosine-protein phosphatase kappa ELISA Kit;Human dJ480J14.2.1 (protein tyrosine phosphatase, receptor type, K (R-PTP-KAPPA, protein tyrosine phosphatase kappa , protein tyrosine phosphatase kappa ELISA Kit;Human protein-tyrosine phosphatase, receptor type, kappa ELISA Kit;
  • Catalog
    E032282
  • species
    Human
  • GenePTPRK
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human PTPRK. No significant cross-reactivity or interference between Human PTPRK and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human PTPRK and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman PTPRK ELISA Kit allows for the in vitro quantitative determination of PTPRK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PTPRK in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PTPRK. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PTPRK and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PTPRK, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PTPRK. You can calculate the concentration of Human PTPRK in the samples by comparing the OD of the samples to the standard curve.
  • Human Receptor-type tyrosine-protein phosphatase kappa Protein information
  • Uniprot ID PTPRK_HUMAN
  • Uniprot AC LCGETAIP VCEFKAAYFD MIRIDSQTNS SHLKDEFQTL NSVTPRLQAE DCSIACLPRN
  • UniGene Hs.155919;
  • GeneID 5796
  • KEGG hsa:5796;
  • Human Receptor-type tyrosine-protein phosphatase kappa Protein SEQUENCE

  • SEQUENCE 1439 AA; 162102 MW; EE1EA6EA3CCA31ED CRC64;

    MDTTAAAALP AFVALLLLSP WPLLGSAQGQ FSAGGCTFDD GPGACDYHQD

    LYDDFEWVHV SAQEPHYLPP EMPQGSYMIV DSSDHDPGEK ARLQLPTMKE

    NDTHCIDFSY LLYSQKGLNP GTLNILVRVN KGPLANPIWN VTGFTGRDWL

    RAELAVSTFW PNEYQVIFEA EVSGGRSGYI AIDDIQVLSY PCDKSPHFLR

    LGDVEVNAGQ NATFQCIATG RDAVHNKLWL QRRNGEDIPV AQTKNINHRR

    FAASFRLQEV TKTDQDLYRC VTQSERGSGV SNFAQLIVRE PPRPIAPPQL

    LGVGPTYLLI QLNANSIIGD GPIILKEVEY RMTSGSWTET HAVNAPTYKL

    WHLDPDTEYE IRVLLTRPGE GGTGLPGPPL ITRTKCAEPM RTPKTLKIAE

    IQARRIAVDW ESLGYNITRC HTFNVTICYH YFRGHNESKA DCLDMDPKAP

    QHVVNHLPPY TNVSLKMILT NPEGRKESEE TIIQTDEDVP GPVPVKSLQG

    TSFENKIFLN WKEPLDPNGI ITQYEISYSS IRSFDPAVPV AGPPQTVSNL

    WNSTHHVFMH LHPGTTYQFF IRASTVKGFG PATAINVTTN ISAPTLPDYE

    GVDASLNETA TTITVLLRPA QAKGAPISAY QIVVEELHPH RTKREAGAME

    CYQVPVTYQN AMSGGAPYYF AAELPPGNLP EPAPFTVGDN RTYQGFWNPP

    LAPRKGYNIY FQAMSSVEKE TKTQCVRIAT KAATEEPEVI PDPAKQTDRV

    VKIAGISAGI LVFILLLLVV ILIVKKSKLA KKRKDAMGNT RQEMTHMVNA

    MDRSYADQST LHAEDPLSIT FMDQHNFSPR YENHSATAES SRLLDVPRYL

    CEGTESPYQT GQLHPAIRVA DLLQHINLMK TSDSYGFKEE YESFFEGQSA

    SWDVAKKDQN RAKNRYGNII AYDHSRVILQ PVEDDPSSDY INANYIDGYQ

    RPSHYIATQG PVHETVYDFW RMIWQEQSAC IVMVTNLVEV GRVKCYKYWP

    DDTEVYGDFK VTCVEMEPLA EYVVRTFTLE RRGYNEIREV KQFHFTGWPD

    HGVPYHATGL LSFIRRVKLS NPPSAGPIVV HCSAGAGRTG CYIVIDIMLD

    MAEREGVVDI YNCVKALRSR RINMVQTEEQ YIFIHDAILE ACLCGETAIP

    VCEFKAAYFD MIRIDSQTNS SHLKDEFQTL NSVTPRLQAE DCSIACLPRN

    HDKNRFMDML PPDRCLPFLI TIDGESSNYI NAALMDSYRQ PAAFIVTQYP

    LPNTVKDFWR LVYDYGCTSI VMLNEVDLSQ GCPQYWPEEG MLRYGPIQVE

    CMSCSMDCDV INRIFRICNL TRPQEGYLMV QQFQYLGWAS HREVPGSKRS

    FLKLILQVEK WQEECEEGEG RTIIHCLNGG GRSGMFCAIG IVVEMVKRQN

    VVDVFHAVKT LRNSKPNMVE APEQYRFCYD VALEYLESS

  • UCSC uc003qbj.4; human. [Q15262-1];



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