Human PTPN11 ELISA Kit

Alternative name
Human protein-tyrosine phosphatase 1D ELISA Kit;Human PTP-1D ELISA Kit;Human protein-tyrosine phosphatase 2C ELISA Kit;Human PTP-2C ELISA Kit;Human SH-PTP2 ELISA Kit;Human SHP-2 ELISA Kit;Human SHP2 ELISA Kit;Human SH-PTP3 ELISA Kit;Human PTP2C ELISA Kit;Human SHPTP2 ELISA Kit;Human BPTP3 ELISA Kit;Human CFC ELISA Kit;Human JMML ELISA Kit;Human METCDS ELISA Kit;Human NS1 ELISA Kit;Human protein tyrosine phosphatase, non-receptor type 11 ELISA Kit;Human tyrosine-protein phosphatase non-receptor type 11 ELISA Kit;
Catalog
E032275
species
Human
GenePTPN11
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human PTPN11. No significant cross-reactivity or interference between Human PTPN11 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human PTPN11 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.094ng/mL
Intended UseHuman PTPN11 ELISA Kit allows for the in vitro quantitative determination of PTPN11 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PTPN11 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PTPN11. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PTPN11 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PTPN11, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PTPN11. You can calculate the concentration of Human PTPN11 in the samples by comparing the OD of the samples to the standard curve.
Human Tyrosine-protein phosphatase non-receptor type 11 Protein information
Uniprot ID PTN11_HUMAN
Uniprot AC Q06124; A8K1D9; Q96HD7;
UniGene Hs.506852;
GeneID 5781
KEGG hsa:5781;
Human Tyrosine-protein phosphatase non-receptor type 11 Protein SEQUENCE
SEQUENCE 597 AA; 68436 MW; 37E8BFC7ECA2D03F CRC64;
MTSRRWFHPN ITGVEAENLL LTRGVDGSFL ARPSKSNPGD FTLSVRRNGA
VTHIKIQNTG DYYDLYGGEK FATLAELVQY YMEHHGQLKE KNGDVIELKY
PLNCADPTSE RWFHGHLSGK EAEKLLTEKG KHGSFLVRES QSHPGDFVLS
VRTGDDKGES NDGKSKVTHV MIRCQELKYD VGGGERFDSL TDLVEHYKKN
PMVETLGTVL QLKQPLNTTR INAAEIESRV RELSKLAETT DKVKQGFWEE
FETLQQQECK LLYSRKEGQR QENKNKNRYK NILPFDHTRV VLHDGDPNEP
VSDYINANII MPEFETKCNN SKPKKSYIAT QGCLQNTVND FWRMVFQENS
RVIVMTTKEV ERGKSKCVKY WPDEYALKEY GVMRVRNVKE SAAHDYTLRE
LKLSKVGQAL LQGNTERTVW QYHFRTWPDH GVPSDPGGVL DFLEEVHHKQ
ESIMDAGPVV VHCSAGIGRT GTFIVIDILI DIIREKGVDC DIDVPKTIQM
VRSQRSGMVQ TEAQYRFIYM AVQHYIETLQ RRIEEEQKSK RKGHEYTNIK
YSLADQTSGD QSPLPPCTPT PPCAEMREDS ARVYENVGLM QQQKSFR
UCSC uc001ttw.2; human. [Q06124-1];

Category

Human PTPN11 ELISA Kit