Human PPOX ELISA Kit

Alternative name
Human PPO ELISA Kit;Human V290M ELISA Kit;Human VP ELISA Kit;Human protoporphyrinogen oxidase ELISA Kit;
Catalog
E032212
species
Human
GenePPOX
SpecificityThis assay has high sensitivity and excellent specificity for detection of Protoporphyrinogen Oxidase (PPOX). No significant cross-reactivity or interference between Protoporphyrinogen Oxidase (PPOX) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.053ng/mL.
Intended UseHuman PPOX ELISA Kit allows for the in vitro quantitative determination of PPOX , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protoporphyrinogen Oxidase (PPOX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protoporphyrinogen Oxidase (PPOX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protoporphyrinogen Oxidase (PPOX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protoporphyrinogen Oxidase (PPOX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Protoporphyrinogen oxidase Protein information
Uniprot ID PPOX_HUMAN
Uniprot AC P50336; D3DVG0; Q5VTW8;
UniGene Hs.517373;
GeneID 5498
KEGG hsa:5498;
Human Protoporphyrinogen oxidase Protein SEQUENCE
SEQUENCE 477 AA; 50765 MW; 2444DEAC2E6C33EE CRC64;
MGRTVVVLGG GISGLAASYH LSRAPCPPKV VLVESSERLG GWIRSVRGPN
GAIFELGPRG IRPAGALGAR TLLLVSELGL DSEVLPVRGD HPAAQNRFLY
VGGALHALPT GLRGLLRPSP PFSKPLFWAG LRELTKPRGK EPDETVHSFA
QRRLGPEVAS LAMDSLCRGV FAGNSRELSI RSCFPSLFQA EQTHRSILLG
LLLGAGRTPQ PDSALIRQAL AERWSQWSLR GGLEMLPQAL ETHLTSRGVS
VLRGQPVCGL SLQAEGRWKV SLRDSSLEAD HVISAIPASV LSELLPAEAA
PLARALSAIT AVSVAVVNLQ YQGAHLPVQG FGHLVPSSED PGVLGIVYDS
VAFPEQDGSP PGLRVTVMLG GSWLQTLEAS GCVLSQELFQ QRAQEAAATQ
LGLKEMPSHC LVHLHKNCIP QYTLGHWQKL ESARQFLTAH RLPLTLAGAS
YEGVAVNDCI ESGRQAAVSV LGTEPNS
UCSC uc001fyg.3; human.;

Category

Human PPOX ELISA Kit

Human PPO ELISA Kit;Human V290M ELISA Kit;Human VP ELISA Kit;Human protoporphyrinogen oxidase ELISA Kit;