Human AP12 ELISA Kit

Contact Us
  • Catalog
  • species
  • GeneAP12
  • Standard CurveHuman AP12 ELISA Kit
  • Other Species Mouse AP12 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of AP-12. No significant cross-reactivity or interference between AP-12 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AP-12 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman AP12 ELISA Kit allows for the in vitro quantitative determination of AP12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilySignal Transduction
  • Product Description
    Principle of the assay: AP-12 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AP-12 antibody and an AP-12-HRP conjugate. The assay sample and buffer are incubated together with AP-12-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AP-12 concentration since AP-12 from samples and AP-12-HRP conjugate compete for the anti-AP-12 antibody binding site. Since the number of sites is limited, as more sites are occupied by AP-12 from the sample, fewer sites are left to bind AP-12-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AP-12 concentration in each sample is interpolated from this standard curve.

Related products with Human AP12 ELISA Kit

Inquiry About Human AP12 ELISA Kit If you hope to order it or contact us directly, please contact us via We will get back to you in 12hrs,Thanks!