Human F2R ELISA Kit

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  • Alternative name

    Human PAR-1 ELISA Kit;Human Coagulation factor II receptor ELISA Kit;Human Thrombin receptor ELISA Kit;Human CF2R ELISA Kit;Human PAR1 ELISA Kit;Human TR ELISA Kit;Human HTR ELISA Kit;Human coagulation factor II thrombin receptor ELISA Kit;Human proteinase-activated receptor 1 ELISA Kit;Human protease-activated receptor 1 ELISA Kit;

  • Catalog
    E032020
  • species
    Human
  • GeneF2R
  • Other Species Mouse F2r ELISA KitBovine F2R ELISA KitRat F2r ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.094ng/ml
  • Intended UseHuman F2R ELISA Kit allows for the in vitro quantitative determination of F2R , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human PAR1antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-human PAR1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PAR1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofhuman PAR1can be calculated.
  • Human Proteinase-activated receptor 1 Protein information
  • Uniprot ID PAR1_HUMAN
  • Uniprot AC P25116; Q53XV0; Q96RF7; Q9BUN4;
  • UniGene Hs.482562;
  • GeneID 2149
  • KEGG hsa:2149;
  • Human Proteinase-activated receptor 1 Protein SEQUENCE
  • SEQUENCE 425 AA; 47441 MW; 41B742A99EEC96AB CRC64;

    MGPRRLLLVA ACFSLCGPLL SARTRARRPE SKATNATLDP RSFLLRNPND

    KYEPFWEDEE KNESGLTEYR LVSINKSSPL QKQLPAFISE DASGYLTSSW

    LTLFVPSVYT GVFVVSLPLN IMAIVVFILK MKVKKPAVVY MLHLATADVL

    FVSVLPFKIS YYFSGSDWQF GSELCRFVTA AFYCNMYASI LLMTVISIDR

    FLAVVYPMQS LSWRTLGRAS FTCLAIWALA IAGVVPLLLK EQTIQVPGLN

    ITTCHDVLNE TLLEGYYAYY FSAFSAVFFF VPLIISTVCY VSIIRCLSSS

    AVANRSKKSR ALFLSAAVFC IFIICFGPTN VLLIAHYSFL SHTSTTEAAY

    FAYLLCVCVS SISCCIDPLI YYYASSECQR YVYSILCCKE SSDPSSYNSS

    GQLMASKMDT CSSNLNNSIY KKLLT

  • UCSC uc003ken.5; human.;



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