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Alternative name
Myeloblastin ELISA KIT; AGP7 ELISA KIT; C-ANCA antigen ELISA KIT; Leukocyte proteinase 3 ELISA KIT; PR-3 ELISA KIT; PR3 ELISA KIT; Neutrophil proteinase 4 ELISA KIT; NP-4 ELISA KIT; P29 ELISA KIT; Wegener autoantigenPRTN3 ELISA KIT; MBN ELISA KIT; PR-3 ELISA KIT; PR3 ELISA KIT; NP-4 ELISA KIT
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Catalog
E032013
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species
Human
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GenePR3
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Proteinase 3 (PR3).
No significant cross-reactivity or interference between Proteinase 3 (PR3) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.061ng/mL.
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Intended UseHuman PR3 ELISA Kit allows for the in vitro quantitative determination of PR3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proteinase 3 (PR3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proteinase 3 (PR3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proteinase 3 (PR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proteinase 3 (PR3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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