Human PR3 ELISA Kit

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  • Alternative name

    Myeloblastin ELISA KIT; AGP7 ELISA KIT; C-ANCA antigen ELISA KIT; Leukocyte proteinase 3 ELISA KIT; PR-3 ELISA KIT; PR3 ELISA KIT; Neutrophil proteinase 4 ELISA KIT; NP-4 ELISA KIT; P29 ELISA KIT; Wegener autoantigenPRTN3 ELISA KIT; MBN ELISA KIT; PR-3 ELISA KIT; PR3 ELISA KIT; NP-4 ELISA KIT

  • Catalog
    E032013
  • species
    Human
  • GenePR3
  • Standard CurveHuman PR3 ELISA Kit
  • Other Species Human PR3 ab IgG ELISA KitHuman PR3 Ab-IgG ELISA KitHuman C-ANCA / PR3 IgG ELISA KitHuman PR3 ANCA ELISA KitHuman PR3-ANCA ELISA KitMouse PR3 Ab ELISA KitMouse C-ANCA / PR3 IgG ELISA KitMouse PR3 ELISA KitMouse PR3 AB-IgG ELISA KitMouse PR3-ANCA ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Proteinase 3 (PR3). No significant cross-reactivity or interference between Proteinase 3 (PR3) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.061ng/mL.
  • Intended UseHuman PR3 ELISA Kit allows for the in vitro quantitative determination of PR3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proteinase 3 (PR3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proteinase 3 (PR3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proteinase 3 (PR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proteinase 3 (PR3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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