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Human APLN ELISA Kit

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  • Alternative name

    Human APJ endogenous ligand ELISA Kit;Human APEL ELISA Kit;Human XNPEP2 ELISA Kit;Human apelin ELISA Kit;Human AGTRL1 ligand ELISA Kit;
  • Catalog
    E003201
  • species
    Human
  • GeneAPLN
  • Other Species Mouse Apln ELISA KitBovine APLN ELISA KitRat Apln ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human apelin. No significant cross-reactivity or interference between human apelin and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 31.25 pg/ml
  • Intended UseHuman APLN ELISA Kit allows for the in vitro quantitative determination of APLN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for apelin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any apelin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for apelin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of apelin bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Apelin-13 Protein information
  • Uniprot ID APEL_HUMAN
  • Uniprot AC Q9ULZ1; Q4VY08; Q8WU89;
  • UniGene Hs.303084;
  • GeneID 8862
  • KEGG hsa:8862;
  • Human Apelin-13 Protein SEQUENCE

  • SEQUENCE 77 AA; 8569 MW; 41521E0DBE97BFDA CRC64;

    MNLRLCVQAL LLLWLSLTAV CGGSLMPLPD GNGLEDGNVR HLVQPRGSRN

    GPGPWQGGRR KFRRQRPRLS HKGPMPF

  • UCSC uc004eus.4; human.;


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