Human WNT7A ELISA Kit

Alternative name
Human Wnt family member 7A ELISA Kit;Human protein Wnt-7a ELISA Kit;Human proto-oncogene Wnt7a protein ELISA Kit;Human wingless-type MMTV integration site family, member 7A ELISA Kit;
Catalog
E031931
species
Human
GeneWNT7A
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<18.75pg/ml
Intended UseHuman WNT7A ELISA Kit allows for the in vitro quantitative determination of WNT7A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human WNT7A antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human WNT7A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human WNT7A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human WNT7A can be calculated.
Human Protein Wnt-7a Protein information
Uniprot ID WNT7A_HUMAN
Uniprot AC O00755; Q96H90; Q9Y560;
UniGene Hs.72290;
GeneID 7476
KEGG hsa:7476;
Human Protein Wnt-7a Protein SEQUENCE
SEQUENCE 349 AA; 39005 MW; 259EF506CFCD7AB0 CRC64;
MNRKARRCLG HLFLSLGMVY LRIGGFSSVV ALGASIICNK IPGLAPRQRA
ICQSRPDAII VIGEGSQMGL DECQFQFRNG RWNCSALGER TVFGKELKVG
SREAAFTYAI IAAGVAHAIT AACTQGNLSD CGCDKEKQGQ YHRDEGWKWG
GCSADIRYGI GFAKVFVDAR EIKQNARTLM NLHNNEAGRK ILEENMKLEC
KCHGVSGSCT TKTCWTTLPQ FRELGYVLKD KYNEAVHVEP VRASRNKRPT
FLKIKKPLSY RKPMDTDLVY IEKSPNYCEE DPVTGSVGTQ GRACNKTAPQ
ASGCDLMCCG RGYNTHQYAR VWQCNCKFHW CCYVKCNTCS ERTEMYTCK
UCSC uc003bye.2; human.;

Category

Human WNT7A ELISA Kit

Human Wnt family member 7A ELISA Kit;Human protein Wnt-7a ELISA Kit;Human proto-oncogene Wnt7a protein ELISA Kit;Human wingless-type MMTV integration site family, member 7A ELISA Kit;