Human WNT10A ELISA Kit

Alternative name
Human OODD ELISA Kit;Human SSPS ELISA Kit;Human STHAG4 ELISA Kit;Human Wnt family member 10A ELISA Kit;Human protein Wnt-10a ELISA Kit;Human wingless-type MMTV integration site family, member 10A ELISA Kit;
Catalog
E031914
species
Human
GeneWNT10A
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094ng/ml
Intended UseHuman WNT10A ELISA Kit allows for the in vitro quantitative determination of WNT10A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human WNT10A antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human WNT10A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human WNT10A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human WNT10A can be calculated.
Human Protein Wnt-10a Protein information
Uniprot ID WN10A_HUMAN
Uniprot AC Q9GZT5; Q53S44; Q96TA7; Q9H7S8;
UniGene Hs.121540;
GeneID 80326
KEGG hsa:80326;
Human Protein Wnt-10a Protein SEQUENCE
SEQUENCE 417 AA; 46444 MW; 868DF5146A895319 CRC64;
MGSAHPRPWL RLRPQPQPRP ALWVLLFFLL LLAAAMPRSA PNDILDLRLP
PEPVLNANTV CLTLPGLSRR QMEVCVRHPD VAASAIQGIQ IAIHECQHQF
RDQRWNCSSL ETRNKIPYES PIFSRGFRES AFAYAIAAAG VVHAVSNACA
LGKLKACGCD ASRRGDEEAF RRKLHRLQLD ALQRGKGLSH GVPEHPALPT
ASPGLQDSWE WGGCSPDMGF GERFSKDFLD SREPHRDIHA RMRLHNNRVG
RQAVMENMRR KCKCHGTSGS CQLKTCWQVT PEFRTVGALL RSRFHRATLI
RPHNRNGGQL EPGPAGAPSP APGAPGPRRR ASPADLVYFE KSPDFCEREP
RLDSAGTVGR LCNKSSAGSD GCGSMCCGRG HNILRQTRSE RCHCRFHWCC
FVVCEECRIT EWVSVCK
UCSC uc002vjd.2; human.;

Category

Human WNT10A ELISA Kit

Human OODD ELISA Kit;Human SSPS ELISA Kit;Human STHAG4 ELISA Kit;Human Wnt family member 10A ELISA Kit;Human protein Wnt-10a ELISA Kit;Human wingless-type MMTV integration site family, member 10A ELISA Kit;