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  • Alternative name

    Receptor-type tyrosine-protein phosphatase F ELISA KIT; Leukocyte common antigen related ELISA KIT; LARPTPRF ELISA KIT; LAR ELISA KIT; LAR ELISA KIT

  • Catalog
  • species
  • GenePTPRF
  • Standard CurveHuman PTPRF ELISA Kit
  • Other Species Mouse Ptprf ELISA KitBovine PTPRF ELISA KitRat Ptprf ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PTPRF. No significant cross-reactivity or interference between PTPRF and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of PTPRF is typically less than 0.31ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two
  • Intended UseHuman PTPRF ELISA Kit allows for the in vitro quantitative determination of PTPRF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PTPRF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to PTPRF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PTPRF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PTPRF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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