Human QKI ELISA Kit

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  • Alternative name

    Human Hqk ELISA Kit;Human hqkI ELISA Kit;Human HKQ ELISA Kit;Human QK ELISA Kit;Human QK1 ELISA Kit;Human QK3 ELISA Kit;Human QKI, KH domain containing RNA binding ELISA Kit;Human protein quaking ELISA Kit;Human RNA binding protein HQK ELISA Kit;Human homolog of mouse quaking QKI (KH domain RNA binding protein) ELISA Kit;Human quaking homolog, KH domain RNA binding ELISA Kit;

  • Catalog
    E031768
  • species
    Human
  • GeneQKI
  • Standard CurveHuman QKI ELISA Kit
  • Other Species Mouse Qki ELISA KitBovine QKI ELISA KitCanine QKI ELISA KitChicken QKI ELISA KitHorse QKI ELISA KitPorcine QKI ELISA KitRat Qki ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of QKI. No significant cross-reactivity or interference between QKI and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between QKI and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman QKI ELISA Kit allows for the in vitro quantitative determination of QKI , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses: This QKI ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human QKI. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: QKI ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for QKI. Standards or samples are then added to the microtiter plate wells and QKI if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of QKI present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for QKI are added to each well to "sandwich" the QKI immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain QKI and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The QKI concentration in each sample is interpolated from this standard curve.
  • Human Protein quaking Protein information
  • Uniprot ID QKI_HUMAN
  • Uniprot AC Q9P0X6; Q9P0X7; Q9P0X8; Q9P0X9; Q9P0Y0; Q9P0Y1;
  • UniGene Hs.510324;
  • GeneID 9444
  • KEGG hsa:9444;
  • Human Protein quaking Protein SEQUENCE
  • SEQUENCE 341 AA; 37671 MW; 43E7F3A426A494C4 CRC64;

    MVGEMETKEK PKPTPDYLMQ LMNDKKLMSS LPNFCGIFNH LERLLDEEIS

    RVRKDMYNDT LNGSTEKRSA ELPDAVGPIV QLQEKLYVPV KEYPDFNFVG

    RILGPRGLTA KQLEAETGCK IMVRGKGSMR DKKKEEQNRG KPNWEHLNED

    LHVLITVEDA QNRAEIKLKR AVEEVKKLLV PAAEGEDSLK KMQLMELAIL

    NGTYRDANIK SPALAFSLAA TAQAAPRIIT GPAPVLPPAA LRTPTPAGPT

    IMPLIRQIQT AVMPNGTPHP TAAIVPPGPE AGLIYTPYEY PYTLAPATSI

    LEYPIEPSGV LGAVATKVRR HDMRVHPYQR IVTADRAATG N

  • UCSC uc003que.4; human. [Q96PU8-1];


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