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Human PPM1A ELISA Kit

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  • Alternative name

    Human Protein phosphatase 2C isoform alpha ELISA Kit;Human PP2C-ALPHA ELISA Kit;Human Protein phosphatase IA ELISA Kit;Human PPPM1A ELISA Kit;Human PP2CA ELISA Kit;Human PP2Calpha ELISA Kit;Human protein phosphatase, Mg2+/Mn2+ dependent 1A ELISA Kit;Human protein phosphatase 1A ELISA Kit;Human protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform ELISA Kit;
  • Catalog
    E031765
  • species
    Human
  • GenePPM1A
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A). No significant cross-reactivity or interference between Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.123ng/mL.
  • Intended UseHuman PPM1A ELISA Kit allows for the in vitro quantitative determination of PPM1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Protein phosphatase 1A Protein information
  • Uniprot ID PPM1A_HUMAN
  • Uniprot AC P35813; B5BU11; J3KNM0; O75551;
  • UniGene Hs.130036;
  • GeneID 5494
  • KEGG hsa:5494;
  • Human Protein phosphatase 1A Protein SEQUENCE

  • SEQUENCE 382 AA; 42448 MW; D48EF508B4A76687 CRC64;

    MGAFLDKPKM EKHNAQGQGN GLRYGLSSMQ GWRVEMEDAH TAVIGLPSGL

    ESWSFFAVYD GHAGSQVAKY CCEHLLDHIT NNQDFKGSAG APSVENVKNG

    IRTGFLEIDE HMRVMSEKKH GADRSGSTAV GVLISPQHTY FINCGDSRGL

    LCRNRKVHFF TQDHKPSNPL EKERIQNAGG SVMIQRVNGS LAVSRALGDF

    DYKCVHGKGP TEQLVSPEPE VHDIERSEED DQFIILACDG IWDVMGNEEL

    CDFVRSRLEV TDDLEKVCNE VVDTCLYKGS RDNMSVILIC FPNAPKVSPE

    AVKKEAELDK YLECRVEEII KKQGEGVPDL VHVMRTLASE NIPSLPPGGE

    LASKRNVIEA VYNRLNPYKN DDTDSTSTDD MW

  • UCSC uc001xew.5; human. [P35813-1];



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