Human PPP1CB ELISA Kit

Alternative name
Human PP-1B ELISA Kit;Human PPP1CD ELISA Kit;Human HEL-S-80p ELISA Kit;Human PP1B ELISA Kit;Human PP1beta ELISA Kit;Human protein phosphatase 1 catalytic subunit beta ELISA Kit;Human serine/threonine-protein phosphatase PP1-beta catalytic subunit ELISA Kit;Human epididymis secretory sperm binding protein Li 80p ELISA Kit;Human protein phosphatase 1, catalytic subunit, beta isoform ELISA Kit;Human protein phosphatase 1, catalytic subunit, beta isozyme ELISA Kit;Human protein phosphatase 1, catalytic subunit, delta isoform ELISA Kit;Human protein phosphatase 1-beta ELISA Kit;Human protein phosphatase 1-delta ELISA Kit;
Catalog
E031705
species
Human
GenePPP1CB
SpecificityThis assay has high sensitivity and excellent specificity for detection of human PPP1CB. No significant cross-reactivity or interference between human PPP1CB and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human PPP1CB is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman PPP1CB ELISA Kit allows for the in vitro quantitative determination of PPP1CB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PPP1CB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PPP1CB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPP1CB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPP1CB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Serine/threonine-protein phosphatase PP1-beta catalytic subunit Protein information
Uniprot ID PP1B_HUMAN
Uniprot AC P62140; B2R5V4; D6W565; P37140; Q5U087; Q6FG45;
UniGene Hs.702907;
GeneID 5500
KEGG hsa:5500;
Human Serine/threonine-protein phosphatase PP1-beta catalytic subunit Protein SEQUENCE
SEQUENCE 327 AA; 37187 MW; E8356022E9B94ECD CRC64;
MADGELNVDS LITRLLEVRG CRPGKIVQMT EAEVRGLCIK SREIFLSQPI
LLELEAPLKI CGDIHGQYTD LLRLFEYGGF PPEANYLFLG DYVDRGKQSL
ETICLLLAYK IKYPENFFLL RGNHECASIN RIYGFYDECK RRFNIKLWKT
FTDCFNCLPI AAIVDEKIFC CHGGLSPDLQ SMEQIRRIMR PTDVPDTGLL
CDLLWSDPDK DVQGWGENDR GVSFTFGADV VSKFLNRHDL DLICRAHQVV
EDGYEFFAKR QLVTLFSAPN YCGEFDNAGG MMSVDETLMC SFQILKPSEK
KAKYQYGGLN SGRPVTPPRT ANPPKKR

Category

Human PPP1CB ELISA Kit