Human LIN28A ELISA Kit

Alternative name
Human lin-28A ELISA Kit;Human zinc finger CCHC domain-containing protein 1 ELISA Kit;Human CSDD1 ELISA Kit;Human LIN28 ELISA Kit;Human ZCCHC1 ELISA Kit;Human LIN-28 ELISA Kit;Human lin-28 homolog A ELISA Kit;Human protein lin-28 homolog A ELISA Kit;Human RNA-binding protein LIN-28 ELISA Kit;Human zinc finger, CCHC domain containing 1 ELISA Kit;
Catalog
E031669
species
Human
GeneLIN28A
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094ng/ml
Intended UseHuman LIN28A ELISA Kit allows for the in vitro quantitative determination of LIN28A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human LIN28A antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human LIN28A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human LIN28A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human LIN28A can be calculated.
Human Protein lin-28 homolog A Protein information
Uniprot ID LN28A_HUMAN
Uniprot AC Q9H9Z2;
UniGene Hs.86154;
GeneID 79727
KEGG hsa:79727;
Human Protein lin-28 homolog A Protein SEQUENCE
SEQUENCE 209 AA; 22743 MW; FA5EF6DD33FABF54 CRC64;
MGSVSNQQFA GGCAKAAEEA PEEAPEDAAR AADEPQLLHG AGICKWFNVR
MGFGFLSMTA RAGVALDPPV DVFVHQSKLH MEGFRSLKEG EAVEFTFKKS
AKGLESIRVT GPGGVFCIGS ERRPKGKSMQ KRRSKGDRCY NCGGLDHHAK
ECKLPPQPKK CHFCQSISHM VASCPLKAQQ GPSAQGKPTY FREEEEEIHS
PTLLPEAQN
UCSC uc001bmj.4; human.;

Human LIN28A ELISA Kit

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