Human PRKAB1 ELISA Kit

Alternative name
Human AMPK subunit beta-1 ELISA Kit;Human AMPKb ELISA Kit;Human AMPK ELISA Kit;Human HAMPKb ELISA Kit;Human protein kinase AMP-activated non-catalytic subunit beta 1 ELISA Kit;Human 5'-AMP-activated protein kinase subunit beta-1 ELISA Kit;Human 5'-AMP-activated protein kinase beta-1 subunit ELISA Kit;Human AMP-activated protein kinase beta subunit ELISA Kit;Human AMPK beta -1 chain ELISA Kit;Human AMPK beta 1 ELISA Kit;Human protein kinase, AMP-activated, beta 1 non-catalytic subunit ELISA Kit;Human protein kinase, AMP-activated, noncatalytic, beta-1 ELISA Kit;
Catalog
E031653
species
Human
GenePRKAB1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Protein Kinase, AMP Activated Beta 1 (PRKAb1). No significant cross-reactivity or interference between Protein Kinase, AMP Activated Beta 1 (PRKAb1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.124ng/mL.
Intended UseHuman PRKAB1 ELISA Kit allows for the in vitro quantitative determination of PRKAB1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase, AMP Activated Beta 1 (PRKAb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase, AMP Activated Beta 1 (PRKAb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase, AMP Activated Beta 1 (PRKAb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase, AMP Activated Beta 1 (PRKAb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human 5'-AMP-activated protein kinase subunit beta-1 Protein information
Uniprot ID AAKB1_HUMAN
Uniprot AC Q9Y478; Q9UBV0; Q9UE20; Q9UEX2; Q9Y6V8;
UniGene Hs.741184;
GeneID 5564
KEGG hsa:5564;
Human 5'-AMP-activated protein kinase subunit beta-1 Protein SEQUENCE
SEQUENCE 270 AA; 30382 MW; F0BCAA94D5BC15FC CRC64;
MGNTSSERAA LERHGGHKTP RRDSSGGTKD GDRPKILMDS PEDADLFHSE
EIKAPEKEEF LAWQHDLEVN DKAPAQARPT VFRWTGGGKE VYLSGSFNNW
SKLPLTRSHN NFVAILDLPE GEHQYKFFVD GQWTHDPSEP IVTSQLGTVN
NIIQVKKTDF EVFDALMVDS QKCSDVSELS SSPPGPYHQE PYVCKPEERF
RAPPILPPHL LQVILNKDTG ISCDPALLPE PNHVMLNHLY ALSIKDGVMV
LSATHRYKKK YVTTLLYKPI
UCSC uc001txg.4; human.;

Category

Human PRKAB1 ELISA Kit