Human PRKAB1 ELISA Kit

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  • Alternative name

    Human AMPK subunit beta-1 ELISA Kit;Human AMPKb ELISA Kit;Human AMPK ELISA Kit;Human HAMPKb ELISA Kit;Human protein kinase AMP-activated non-catalytic subunit beta 1 ELISA Kit;Human 5'-AMP-activated protein kinase subunit beta-1 ELISA Kit;Human 5'-AMP-activated protein kinase beta-1 subunit ELISA Kit;Human AMP-activated protein kinase beta subunit ELISA Kit;Human AMPK beta -1 chain ELISA Kit;Human AMPK beta 1 ELISA Kit;Human protein kinase, AMP-activated, beta 1 non-catalytic subunit ELISA Kit;Human protein kinase, AMP-activated, noncatalytic, beta-1 ELISA Kit;

  • Catalog
    E031653
  • species
    Human
  • GenePRKAB1
  • Standard CurveHuman PRKAB1 ELISA Kit
  • Other Species Mouse Prkab1 ELISA KitBovine PRKAB1 ELISA KitPorcine PRKAB1 ELISA KitRat Prkab1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Protein Kinase, AMP Activated Beta 1 (PRKAb1). No significant cross-reactivity or interference between Protein Kinase, AMP Activated Beta 1 (PRKAb1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.124ng/mL.
  • Intended UseHuman PRKAB1 ELISA Kit allows for the in vitro quantitative determination of PRKAB1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase, AMP Activated Beta 1 (PRKAb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase, AMP Activated Beta 1 (PRKAb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase, AMP Activated Beta 1 (PRKAb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase, AMP Activated Beta 1 (PRKAb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human 5'-AMP-activated protein kinase subunit beta-1 Protein information
  • Uniprot ID AAKB1_HUMAN
  • Uniprot AC Q9Y478; Q9UBV0; Q9UE20; Q9UEX2; Q9Y6V8;
  • UniGene Hs.741184;
  • GeneID 5564
  • KEGG hsa:5564;
  • Human 5'-AMP-activated protein kinase subunit beta-1 Protein SEQUENCE
  • SEQUENCE 270 AA; 30382 MW; F0BCAA94D5BC15FC CRC64;

    MGNTSSERAA LERHGGHKTP RRDSSGGTKD GDRPKILMDS PEDADLFHSE

    EIKAPEKEEF LAWQHDLEVN DKAPAQARPT VFRWTGGGKE VYLSGSFNNW

    SKLPLTRSHN NFVAILDLPE GEHQYKFFVD GQWTHDPSEP IVTSQLGTVN

    NIIQVKKTDF EVFDALMVDS QKCSDVSELS SSPPGPYHQE PYVCKPEERF

    RAPPILPPHL LQVILNKDTG ISCDPALLPE PNHVMLNHLY ALSIKDGVMV

    LSATHRYKKK YVTTLLYKPI

  • UCSC uc001txg.4; human.;


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